Single cell RNAseq of adoptively transferred Tregs in rhesus macaques

ABSTRACT: Animal R.401 was infused with ex-vivo expanded T-regulatory cells. After adoptive transfer, PBMC were obtained and stained with surface antibodies. Single cell libraries from sorted populations were generated using a UMI-based droplet-partitioning platform (10X Genomics) and sequenced using a NextSeq 500 (Illumina). Resulting reads were processed using Cellranger software (10X Genomics) and downstream analysis was performed using Monocle6,7 using a negative binomial model of distribution with fixed variance.

ORGANISM(S): Macaca mulatta

PROVIDER: GSE139200 | GEO | 2019/10/24

REPOSITORIES: GEO