Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromatin binding protein PHF6 regulates activity-dependent transcription networks to promote hunger response [RNA-Seq]

ABSTRACT: We revealed that PHF6 bound to the promoters of a subset of neuronal immediate-early genes (IEGs) and regulate their expression levels. hunger state.

ORGANISM(S): Mus musculus

PROVIDER: GSE139543 | GEO | 2020/03/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Chromatin binding protein PHF6 regulates activity-dependent transcription networks to promote hunger response [ChIP-Seq]

Project description:We revealed that PHF6 was bound to the promoters of a subset of neuronal immediate-early genes (IEGs), and this chromatin binding was dynamically regulated by hunger state.

2020-03-16 | GSE133901 | GEO

Chromatin binding protein PHF6 regulates activity-dependent transcription networks to promote hunger response

Project description:Chromatin binding protein PHF6 regulates activity-dependent transcription networks to promote hunger response

| PRJNA580227 | ENA

Chromatin binding protein PHF6 regulates activity-dependent transcription networks to promote hunger response [ChIP-Seq]

Project description:Chromatin binding protein PHF6 regulates activity-dependent transcription networks to promote hunger response [ChIP-Seq]

| PRJNA552959 | ENA

Chromatin binding protein PHF6 regulates activity-dependent transcription networks to promote hunger response [RNA-Seq]

Project description:Chromatin binding protein PHF6 regulates activity-dependent transcription networks to promote hunger response [RNA-Seq]

| PRJNA580230 | ENA

Chromatin binding protein PHF6 regulates activity-dependent transcription networks to promote hunger response

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-16 | GSE139545 | GEO

PHF6 and JAK3 mutations cooperate to drive T-cell acute lymphoblastic leukemia progression (ChIP-Seq)

Project description:Chromatin immunoprecipitation sequencing (ChIP-seq) analysis of HA-PHF6-overexpressing (PHF6 OE) K562 cells showed that PHF6 directly bound to the ADGRB1 (BAI1) gene

2022-02-09 | GSE159549 | GEO

RNA sequencing of isogenic Phf6 wild type and Phf6 knockout mouse T-ALL cells

Project description:Loss-of-function mutations Phf6 occur frequently in both adult and pediatric T-cell Acute Lymphoblastic Leukemia (T-ALL). Although Phf6 is widely expressed, little is known about its proposed function as epigenetic regulator and tumor suppressor. To address the role of Phf6 in the leukemia development, here we analyze by RNAseq the gene expression profiles of isogenic Phf6 wild type and Phf6 knockout leukemia cells transformed by overexpression of an oncogenic mutant form of the NOTCH1 receptor.

2019-07-31 | GSE117165 | GEO

PHF6 OCCUPANCYIN HUMAN T-CELL ACUTE LYMPHOBLASTIC LEUKEMIA

Project description:Here we identify the genome-wide occupancy of PHF6 in T-ALL cells. Human T-ALL cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total PHF6 (Bethyl A301-451A). This represents the ChIP-seq portion of this dataset.

2014-04-01 | E-GEOD-45864 | biostudies-arrayexpress

PHF6 knockdown in rat cortical neurons

Project description:E18 embryonic rat cortical neurons cultured in vitro are infected with lentivirus expressing control or PHF6shRNA-2, and harvested 5 days after infection pLL3.7 lentivirus expressing control or PHF6shRNA-2 was generated in 293T cells and concentrated using ultracentrifuge. In vitro cultured cortical neurons were infected and RNA was harvested 5 days after infection. PHF6 knockdown was validated by QPCR before sample was processed for microarray analysis.

2013-08-01 | E-GEOD-45953 | biostudies-arrayexpress

AgRP neuron epigenomes across hunger states reveal that IRF3 mediates leptin's effects

Project description:AgRP neurons in the arcuate nucleus of the hypothalamus (ARC) coordinate homeostatic changes in appetite associated with fluctuations in food availability and leptin signaling. Identifying the relevant transcriptional regulatory pathways in these neurons has been a priority, yet such attempts have been stymied due to their low abundance and the rich cellular diversity of the ARC. Here we generated male mouse AgRP neuron-specific transcriptomic and chromatin accessibility profiles during three distinct hunger states of satiety, fasting-induced hunger, and leptin-induced hunger suppression. Cis-regulatory analysis of these integrated datasets enabled the identification of 28 putative hunger-promoting and 29 putative hunger-suppressing transcriptional regulators in AgRP neurons, 16 of which were predicted to be transcriptional effectors of leptin. Within our dataset, Interferon regulatory factor 3 (IRF3) emerged as a leading candidate mediator of leptin-induced hunger-suppression. Gain- and loss-of-function experiments in vivo confirm the role of IRF3 in mediating the acute satiety-evoking effects of leptin in AgRP neurons, while live-cell imaging in vitro indicate that leptin can activate neuronal IRF3 in a cell autonomous manner. Finally, we employ CUT&RUN to uncover direct transcriptional targets of IRF3 in AgRP neurons in vivo. Thus, our findings identify AgRP neuron-expressed IRF3 as a key transcriptional effector of the hunger-suppressing effects of leptin.

2024-04-12 | GSE240484 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data