Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from mouse macrophages after being cultured on different surfaces

ABSTRACT: The adverse macrophage-mediated immune response elicited by the bioinert surface of polyetheretherketone (PEEK) is responsible for the formation of fibrous encapsulation and inferior osseointegration for PEEK implants in the dental and orthopedic fields. A nanoporous thin film constructed by layer-by-layer technique was deposited on PEEK surface, and it can effectively suppress the acute inflammatory response of macrophages. However, the underlying molecular mechanism why macrophages respond differently on the modified surface is unknown. We used microarrays to elucidate the underlying molecular mechanisms why macrophages respond differently on the modified surface of PEEK.

ORGANISM(S): Mus musculus

PROVIDER: GSE139591 | GEO | 2019/10/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Analyses of the brown stain on the Parthenon Centaur head

Project description:Marbles of Greek origin have been reported to form a brown stain layer, also known as patina or film. Early investigations discovered that this layer is formed by the two calcium oxalate minerals whewellite and weddellite. The origin of this oxalate film is, however, not clear, while one of the hypotheses is that it is a result of bacteria, fungi, or lichen growing on the surface of the marble. The present study characterizes the brown stain from one of the Parthenon marbles in Danish custody using LC-MS-based proteomics aiming to shed light on the possible biological origin of the staining layer. The marbles curated in Copenhagen are particularly interesting because the pieces have been kept indoors since before the onset of the industrial revolution, therefore they have experienced air pollution to a much lesser degree than the marbles still residing at the Acropolis.

2024-01-17 | PXD043505 | Pride

Mus musculus

Project description:Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis

| PRJNA348679 | ENA

Macrophages from newly hatched chicks [set2]

2014-07-31 | GSE59920 | GEO

Intra-surgical Protein Layer on Titanium Arthroplasty Explants

Project description:Aseptic loosening in total joint replacement due to insufficient osteointegration is an unsolved problem in orthopaedics.The purpose of our study was to obtain a picture of the initial protein adsorption layer on femoral endoprosthetic surfaces as the key to the initiation of osseointegration.

2019-04-17 | PXD012614 | Pride

Macrophages from newly hatched chicks [set2]

Project description:To identify markers associated with inherent cellular sex-identity, we analysed macrophages from newly-hatched chicks. We found that male and female macrophages respond differently to stimulation by bacterial lipopolysaccharide and that female macrophages constitutively express higher levels of interferon target genes than male macrophages. Macrophages were collected from leg-bones of chickens between 1 and 3 days after hatch. Three pools of macrophage cells were made for male and female cultures. Cells were cultured in either standard medium or in medium containing lipopolysaccharide (LPS) to activate the macrophages. Macrophages were harvested and RNA collected for microarray analysis.

2014-07-31 | E-GEOD-59920 | biostudies-arrayexpress

Transcription profiling of tomato - regeneration of epicuticular fruit wax

Project description:Mature green fruits of the tomato (Solanum lycopersicum L.) cultivar MicroTom were investigated (fruit developmental category II). For removal of the epicuticular wax layer a thin film of 1 g ml-1 aqueous gum arabic (Roth, Karlsruhe, Germany) as a fixative was applied to the fruit surface. After 1 h the dried polymer including the outermost epicuticular waxes was mechanically removed. This procedure was repeated once. During this experiment the tomato fruits remained on the tomato plant. Untreated (0 days) and gum arabic stripped tomato fruits harvested 2 days after treatment were compared. Samples contained exclusively the fruit peel, which was removed with a scalpel to a depth of approximately 1 mm. After sampling point the plant material was immediately frozen in liquid nitrogen and stored at -80 C until use.

2009-06-09 | E-MEXP-2163 | biostudies-arrayexpress

Genome wide array analysis of endosseous implant adherent cellular phenotypes

Project description:Objective: to identify the early molecular processes involved in osseointegration associated with a micro roughened and nanosurface featured implants. Thirty-two titanium implants with surface topographies exhibiting nanoscale features (AT-I) and microrough surface without nanoscale features (AT-II) were placed in the tibiae of 8 rats and subsequently harvested at 2 and 4 days after placement. Total RNA was isolated from cells adherent to retrieved implants. A whole genome microarray using the Affymetrix Rat gene 1.1 ST Array was used to describe the gene expression profiles that were differentially regulated by the implant surfaces.

2012-09-27 | E-GEOD-35976 | biostudies-arrayexpress

Roles of proteome dynamics and cytokinin signaling in root-to-hypocotyl ratio changes induced by shading roots of Arabidopsis seedlings

Project description:Seeds of Arabidopsis thaliana ecotype Columbia (Col-0) were surface-sterilized (using 75 % ethanol) and sown on Murashige and Skoog medium (pH 5.7) supplemented with 3 mM morpholinoethane sulfonic acid, solidified with 1% (w/v) agar and stratified at 4 °C for 48 h. Petri dishes were covered by a layer of black stretch shrink wrap film (black foil) and aluminum foil and incubated at 21°C/19°C day/night temperatures, with a 16 h photoperiod (photosynthetic photon fluence rate of 20 μmol m-2 s-1 over the waveband 400-700 nm) for 7 d in an AR-36L growth chamber

2015-02-23 | PXD001612 | Pride

Project description:Skin and gut microbiomes respond differently to radionuclide contamination

| PRJEB28050 | ENA

Macrophages from sex-reversed chicken embryos [set1]

Project description:To identify markers associated with inherent cellular sex-identity, we analysed cultured macrophages from male and female chick embryos. We found that male and female macrophages respond differently to stimulation by bacterial lipopolysaccharide and that female macrophages constitutively express higher levels of interferon target genes than male macrophages. To determine whether these differences resulted from the actions of gonadal hormones, we induced gonadal sex-reversal to alter the hormonal environment of the developing chick and analysed different tissues and macrophages from male and female embryos.

2014-07-31 | GSE59919 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data