Sequencing-based quantitative mapping of the cellular small RNA landscape

ABSTRACT: We report the development of an RNA sequencing method – AQRNA-seq – that minimizes biases and enables absolute quantification of all small RNA species in a sample mixture. Validation of AQRNA-seq library preparation and data mining algorithms using a 963-member microRNA reference library, RNA oligonucleotide standards of varying lengths, and northern blots demonstrated a direct, linear correlation between sequencing read count and RNA abundance.

ORGANISM(S): synthetic construct

PROVIDER: GSE139936 | GEO | 2020/01/01

REPOSITORIES: GEO