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ChIP-seq profiling of myogenin and p300 occupancy in the context of rexinoid signaling during early myogenic differentiation

ABSTRACT: Deciphering the molecular mechanisms underpinning myoblast differentiation is a critical step in developing the best strategy to promote muscle regeneration in patients suffering from muscle-related diseases. We have previously established that a rexinoid x receptor (RXR)-selective agonist enhances the differentiation and fusion of myoblasts through a direct regulation of MyoD expression, coupled with an augmentation of myogenin protein. Here, we found that RXR signaling modifies the chromatin state distribution of myogenin, promoting the binding preference of myogenin for poised enhancers and a distinct motif. We also found an association of myogenin with rexinoid-responsive gene expression and identified an epigenetic signature related to histone acetyltransferase p300. Moreover, RXR signaling instigates residue-specific histone acetylation at enhancers co-occupied by p300 and myogenin. Thus, genomic distribution of transcriptional regulators is an important designate for identifying novel targets as well as developing therapeutics that modulate epigenetic landscape in a selective manner to promote muscle regeneration.

ORGANISM(S): Mus musculus

PROVIDER: GSE139942 | GEO | 2020/06/24

REPOSITORIES: GEO

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RNA-seq profiling of rexinoid responsive gene expression during early myogenic differentiation

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2017-09-13 | GSE94560 | GEO

ChIP-seq profiling of myogenin and p300 occupancy in the context of rexinoid signaling during early myogenic differentiation

Project description:ChIP-seq profiling of myogenin and p300 occupancy in the context of rexinoid signaling during early myogenic differentiation

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Evidence for the critical role of retinoid X receptor in rexinoid-induced increase in cellular all-trans-retinoic acid

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MyoD and myogenin ChIP-seq in differentiated C2C12 cells

Project description:The submitted files contain ChIP-seq data for the MyoD and myogenin muscle regulatory factors in diffrentiated C2C12 cells as well as two different sonicated input samples (one from a regular 1% formaldehyde fixation and one from a dual 1%FA + 1.5 mM EGS fix). Characterization of genome-wide MyoD and myogenin binding in C2C12 cells

2013-06-01 | E-GEOD-44824 | biostudies-arrayexpress

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2023-01-11 | GSE199381 | GEO

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2017-09-13 | GSE94558 | GEO

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Project description:Rexinoid signalling during early myogenic differentiation

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Gene Expression Profiling of siRNA Knock-Down of Six1, Six4 and Myogenin in C2C12

Project description:Six1, Six4 and Myogenin are transcription factors that are known to be required for skeletal myogenesis. Currently, very little is known about the genes targeted by Six1 and Six4. Gene expression profiling when one or both transcription factors were knock-down by siRNA was performed to identify genes affected by their absence. We also hypothesized that Six1 and Six4 can work in cooperation with the myogenic regulatory factor (MRFs) family of transcription factors, such as Myogenin. Therefore, we performed the same type of experiment where the myogenin was knocked-down by siRNA to identify genes that are possibly regulated by the Six1 or Six4 in conjunction with Myogenin.

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Comparative expression profiling identifies differential roles for Myogenin and p38α MAPK signaling in myogenesis

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2012-08-02 | GSE25763 | GEO

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