Massively multiplex chemical transcriptomics at single cell resolution

ABSTRACT: Single-cell RNA-seq libraries were generated using two and three level single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) of untreated or small molecule inhibitor exposed HEK293T, NIH3T3, A549, MCF7 and K562 cells. Different cells and different treatment were hashed and pooled prior to sci-RNA-seq using a nuclear barcoding strategy. This nuclear barcoding strategy relies on fixation of barcode containing well-specific oligos that are specific to a given cell type, replicate or treatment condition.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE139944 | GEO | 2019/12/05

REPOSITORIES: GEO