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ChIP-Seq analysis of IbBBX24 transgenic sweet potato plants

ABSTRACT: To better understand the regulatory mechanisms of IbBBX24-mediated Fusarium oxysporum Schlecht. f. sp. batatas (Fob) resistance, we performed ChIP-Seq analysis using overexpression line infected with Fob at 1 dpi.

ORGANISM(S): Ipomoea batatas

PROVIDER: GSE140281 | GEO | 2020/03/04

REPOSITORIES: GEO

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RNA-Seq analysis of IbBBX24 transgenic sweet potato plants

Project description:To better understand the regulatory mechanisms of IbBBX24-mediated Fusarium oxysporum Schlecht. f. sp. batatas (Fob) resistance, we performed RNA-Seq analysis using overexpression line, RNA interference and WT plants infected with Fob at 1 dpi.

2020-03-04 | GSE140181 | GEO

Vanilla planifolia Jacks., transcriptomic response towards Fusarium oxysporum f. sp. vanillae infection

Project description:Upon exposure to unfavorable environmental conditions, plants need to respond quickly to maintain their homeostasis. For instance, physiological, biochemical and transcriptional changes occur during plant-pathogen interaction. In the case of Vanilla planifolia Jacks., a worldwide economically important crop, it is susceptible to Fusarium oxysporum f. sp. vanillae. This pathogen causes root and stem rot in vanilla plants that lead to plant death. To investigate how vanilla plants, respond at the transcriptional level upon infection with F. oxysporum f. sp. vanillae, here we employed the RNA-Seq approach to analyze the dynamics of whole-transcriptome changes during two-time frames of the infection. Analysis of global gene expression profiles indicated that the major transcriptional change occurred at 2 dpi, in comparison to 10 dpi. Whereas 3420 genes were found with a differential expression at 2 dpi, only 839 were identified at 10 dpi. The analysis of the transcriptional profile at 2 dpi suggests that, among other responses, vanilla plants prepare to counter the infection by gathering a pool of translational regulation-related transcripts. The screening of transcriptional changes of V. planifolia Jacks upon infection by F. oxysporum f. sp. vanillae provides insights into the plant molecular response, particularly the upregulation of ribosomal proteins at early stages. Thus, we propose that the plant-pathogen interaction between V. planifolia Jacks and F. oxysporum f. sp. vanillae causes a transcriptional reprogramming coupled with a translational regulation. Altogether, this study provides the identification of molecular players that could help to fight the most damaging disease of vanilla.

2019-11-19 | GSE134155 | GEO

Gene expression after SPVD infection in resistant and susceptible sweet potato cultivars

Project description:Sweet potato virus disease (SPVD) is one of the most devastating diseases affecting sweetpotato (Ipomoea batatas), an important food crop in developing countries. SPVD develops when sweetpotato plants are dually infected with sweet potato feathery mottle virus (SPFMV) and sweet potato chlorotic stunt virus (SPCSV). In the current study, global gene expression between SPVD affected plants and virus-tested control plants (VT) were compared in the susceptible ‘Beauregard’ and resistant ‘NASPOT 1’ (Nas) sweetpotato cultivars at 5, 9, 13 and 17 days post inoculation (DPI).

2010-01-29 | GSE18869 | GEO

Genome Sequencing and Comparative Transcriptomics of Race1 and Race4 of Banana pathogen: Fusarium oxysporum f.sp. Cubense

Project description:Deep sequencing of mRNA from Fusarium oxysporum f. sp. Cubense 1 and 4 after infecting Musa acuminata 0h and 48h.

2014-08-27 | GSE40581 | GEO

Transcriptome analysis reveals the response mechanism of Frl-mediated resistance to Fusarium oxysporum f. sp. radicis-lycopersici (FORL) infection in tomato

Project description:Transcriptome analysis reveals the response mechanism of Frl-mediated resistance to Fusarium oxysporum f. sp. radicis-lycopersici (FORL) infection in tomato

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Microarray expression analysis of transgenic Arabidopsis thaliana

Project description:Comparison of expression differences between Col-0 Arabidopsis thaliana and transgenic plants in the same background carrying three different Fusarium oxysporum effector genes

2017-12-01 | GSE59292 | GEO

Genome Sequencing and Comparative Transcriptomics of Race1 and Race4 of Banana pathogen: Fusarium oxysporum f.sp. Cubense

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2014-08-27 | E-GEOD-40581 | biostudies-arrayexpress

RNA-Seq and ChIP-seq analysis of IbBBX24 transgenic sweet potato plants

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-04 | GSE140283 | GEO

Identification of a DAMP receptor and its cognate peptide ligand in sweet potato (Ipomoea batatas)

Project description:We identified a leucine-rich repeat receptor kinase (IbLRR-RK1) that is induced upon wounding and herbivory, and related to peptide-elicitor receptors (PEPRs) from tomato and Arabidopsis. We also identified a gene encoding a precursor protein comprising a peptide ligand (IbPep1) for IbLRR-RK1. RNAseq of I. batatas reveals differentially expressed genes (DEGs) upon IbPep1 and IbHypSysIV treatment

2023-04-01 | GSE227409 | GEO

RNA-seq-based analysis of the pathogen-induced defense transcriptome in Arabidopsis

Project description:Pathogen infection triggers transcriptional reprogramming in host plants, however we still know little about the dynamics of the pathogen-induced defense transcriptome. The goal of this study was to investigate the dynamic reprogramming of the defense transcriptome in response to Fusarium oxysporum infection in Arabidopsis using RNA-seq technology and to provide a comprehensive analysis of genes underlying the innate immune response against the fungal pathogen. Our results suggest that the Arabidopsis transcriptome is reprogrammed to co-ordinately express multiple positive and negative regulators following pathogen infection to modulate defense gene expression and disease resistance. Our study identified a number of novel genes responsive to pathogen infection and provided a rich source of pathogen responsive genes for further functional characterization. Four samples (M1DPI, M6DPI, F1DPI and F6DPI; M=mock treated; F=Fusarium oxysporum infected; DPI=day post inoculation) were sequenced to identify pathogen responsive genes in each time point. Each sample was sequenced once, i.e. without biological replicate.

2012-06-01 | E-GEOD-34241 | biostudies-arrayexpress

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