Genomics

Dataset Information

44

Gold induction of C. metallidurans CH34


ABSTRACT: Whole-genome expression microarray experiments were conducted to assess the response of C. metallidurans CH34 to aqueous Au(III)-chloride and identify possible biochemical pathways for Au detoxification. Four microarray experiments were conducted to assess gold response at low, medium and high Au(III)-chloride concentrations (i.e., 10, 50 and 100 µM Au(III)-chloride) and different induction times (10 and 30 minutes with 50 µM Au(III)-chloride). Based on these arrays, the differentially expressed genes upon gold-exposure can be identified. Overall design: The four microarray experiments (10, 50 and 100 µM Au(III)-chloride at 10 minutes and 50 µM Au(III)-chloride at 30 minutes) were all performed in biological triplicate and containing three (in-slide) technical repeats. For all conditions, the metal-induced sample (Cy5) was compared with the non-induced sample (Cy5) to identify those genes that were differentially expressed upon gold exposure.

INSTRUMENT(S): SCKCEN Cupriavidus metallidurans CH34 Genomic Array

ORGANISM(S): Cupriavidus metallidurans CH34  

SUBMITTER: Pieter Monsieurs  

PROVIDER: GSE14049 | GEO | 2009-10-29

SECONDARY ACCESSION(S): PRJNA112411

REPOSITORIES: GEO

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