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Transcriptomic profiling in fins of Atlantic salmon parasitized with sea lice: Determining the differentially expressed transcripts in fins of lice-infected salmon at 8 days post-infection

ABSTRACT: Sea lice (e.g., Lepeophtheirus salmonis) are parasites causing costly disease outbreaks in salmon farming globally. Despite the currently available controlling practices, molecular insights into the parasite-induced host immune responses and host-pathogen interaction will provide the basis for improved and innovative treatment strategies. We investigated the early transcriptomic responses in the fins of Atlantic salmon parasitized with sea lice at the chalimus stage (8 days post-infection). Fin tissue collected from non-infected (PRE) control and at (ATT) and adjacent (ADJ) to chalimus-attachment sites from infected fish were used in profiling the global gene expression using a 44K microarray platform. Microarray analyses indicated that global transcriptomic changes resulted in 6,568 differentially expressed probes (DEPs, FDR < 5%) that include 1928 shared DEPs between ATT and ADJ compared to PRE. A direct comparison of ATT vs. ADJ revealed 90 DEPs, all of which were up-regulated in ATT.

ORGANISM(S): Salmo salar

PROVIDER: GSE140756 | GEO | 2020/04/01

REPOSITORIES: GEO

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Interacting effects of sea louse (Lepeophtheirus salmonis) infection and formalin-killed Aeromonas salmonicida in Atlantic salmon skin transcriptome

Project description:Lepeophtheirus salmonis (sea lice) and bacterial co-infection threatens wild and farmed Atlantic salmon performance and welfare. The present microarray-based study examined the dorsal skin transcriptome response to formalin-killed Aeromonas salmonicida bacterin (ASAL) in pre-adult sea lice-infected and non-infected Atlantic salmon to fill the existing knowledge gap and aid in developing anti-co-infection strategies. To this aim, sea lice-infected and non-infected salmon were intraperitoneally injected with either phosphate-buffered saline (PBS) or ASAL (i.e., 4 injection/infection groups: PBS/no lice, PBS/lice, ASAL/no lice, and ASAL/lice). The analysis of the dorsal skin transcriptome data [Significance Analysis of Microarrays (5% FDR)] identified 345 up-regulated and 2,189 down-regulated DEPs in the comparison PBS/lice vs. PBS/no lice, and 82 up-regulated and 3 down-regulated DEPs in the comparison ASAL/lice vs. ASAL/no lice. The comparison ASAL/lice vs. PBS/lice identified 272 up-regulated and 11 down-regulated DEPs, whereas ASAL/no lice vs. PBS/no lice revealed 27 up-regulated DEPs. The skin transcriptome differences between the co-stimulated salmon (i.e., ASAL/lice) and PBS/no lice salmon accounted for 1,878 up-regulated and 3,120 down-regulated DEPs.

2022-02-28 | GSE186292 | GEO

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Transcriptomic profiling in fins of Atlantic salmon parasitized with sea lice: Determining the differentially expressed transcripts in fins of lice-infected salmon at 8 days post-infection

Project description:Transcriptomic profiling in fins of Atlantic salmon parasitized with sea lice: Determining the differentially expressed transcripts in fins of lice-infected salmon at 8 days post-infection

| PRJNA590800 | ENA

Transcriptomic responses to emamectin benzoate in Pacific and Atlantic Canada salmon lice Lepeophtheirus salmonis with differing levels of drug resistance

2014-11-17 | GSE56024 | GEO

Dietary phytochemicals modulate skin gene expression and reduce lice counts after infection in Atlantic salmon

Project description:BACKGROUND: The use of phytochemicals is a promising solution in biological control against salmon louse (Lepeophtheirus salmonis). Glucosinolates (Gls) belong to a diverse group of compounds used as protection against herbivores by plants in the Brassicaceae family, while in vertebrates, ingested glucosinolates exert health-promoting effects due to their antioxidant and detoxifying properties as well as effects on cell proliferation and growth. The aim of this study was to investigate if Atlantic salmon fed two different doses of glucosinolate-enriched feeds would be protected against lice infection. The effects of feeding high dose of glucosinolates before the infection, and of high and low doses 5 weeks into the infection were studied. METHODS: Skin was screened by 15k oligonucleotide microarray and qPCR. RESULTS: 25% reduction (p < 0.05) in lice counts was obtained in the low dose group and 17% reduction in the high dose group compared to fish fed control feed. Microarray analysis revealed induction of over 50 interferon (IFN)-related genes prior to lice infection. Genes upregulated 5 weeks into the infection in glucosinolate-enriched dietary groups included Type 1 pro-inflammatory factors, antimicrobial and acute phase proteins, extracellular matrix remodeling proteases and iron homeostasis regulators. In contrast, genes involved in muscle contraction, lipid and glucose metabolism were found more highly expressed in the skin of infected control fish. CONCLUSIONS: Atlantic salmon fed glucosinolates had a significantly lower number of sea lice at the end of the experimental challenge. Feeding glucosinolates coincided with increased expression of IFN-related genes, and higher expression profiles of Type 1 immune genes late into the infection. In addition, regulation of genes involved in the metabolism of iron, lipid and sugar suggested an interplay between metabolism of nutrients and mechanisms of resistance.

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2016-08-08 | E-GEOD-76555 | biostudies-arrayexpress

Transcriptomic responses to emamectin benzoate in Pacific and Atlantic Canada salmon lice Lepeophtheirus salmonis with differing levels of drug resistance

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2014-11-17 | E-GEOD-56024 | biostudies-arrayexpress

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2017-08-01 | GSE94692 | GEO

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Sex-Biased Gene Expression and Sequence Conservation in Pacific and Atlantic Salmon Lice (Lepeophtheirus salmonis)

Project description:This study investigates sex-biased gene expression between populations of Atlantic and Pacific salmon lice, Lepeophtheirus salmonis. Two Atlantic L. salmonis populations were previously used for an array study (GSE56024) while a third dataset using Pacific L. salmonis was novel. Using all three populations, a consensus-based, meta-analysis approach was used to identify sex-biased and sex-specific genes. Two separate experiments were conducted, one for Atlantic and one for Pacific salmon lice. As the Atlantic data has been previously published for other comparisons (GSE56024), only the Pacific data is uploaded here. Lice from three populations (2 in the Atlantic and 1 in the Pacific) were collected for in vitro bioassay analysis using emamectin benzoate. After 24hrs, lice were collected as per treatment protocol below. Males and females from all populations were compared separately before forming a consensus probe list of sex-biased genes concordantly expressed across all three populations. Please note that each raw data file contains three or four 'block' data and each block data correspond to individual sample raw data. Therefore, each raw data file contains raw data for 3-4 samples (as indicated in the description field).

2016-06-01 | E-GEOD-73734 | biostudies-arrayexpress

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