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ASLV vector infected 293t-TVA cells

ABSTRACT: Human 293T cells engineered to express the TVA receptor (see Mitchell et al., PLoS) infected with an ASLV vector. RNA isolated 48 hours later. ***PLEASE NOTE*** Series entries GSE1408 & GSE1409 no longer exist. All data discussed in the author's PLoS publication (PubMed ID 15314653) is available under GSE1407 & GSE1410. ***PLEASE NOTE*** Keywords: repeat sample

ORGANISM(S): Homo sapiens

PROVIDER: GSE1410 | GEO | 2004/05/19

SECONDARY ACCESSION(S): PRJNA90085

REPOSITORIES: GEO

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Transcription profiling of human 293 T cells infected with ASLV

Project description:Human 293T cells engineered to express the TVA receptor (see Mitchell et al., PLoS) infected with an ASLV vector. RNA isolated 48 hours later. ***PLEASE NOTE***; Series entries GSE1408 & GSE1409 no longer exist. All data discussed in the author's PLoS publication (PubMed ID 15314653) is available under GSE1407 & GSE1410. ***PLEASE NOTE***

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Project description:To investigate the role of Creb1 in TVA dependent CD8+ T cell activation, we knocked down Creb1 of mouse CD8+ T cells with siRNAs then treated with DMSO or TVA We then performed gene expression profiling analysis using data obtained from RNA-seq.

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Effect of trans-vaccenic acid(TVA) diet on 16S ribosomal RNA (rRNA) expression of mouse gut microbiome

Project description:To investigate the TVA diet's effect on mouse gut microbiome, we fed C57/BL6 mice with TVA diet or CON diet for 18 days We then collected feces of the mice and performed 16S ribosomal RNA (rRNA) sequencing.

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Homo sapiens

Project description:ASLV vector infected 293t-TVA cells

| PRJNA90085 | ENA

Effect of trans-vaccenic acid(TVA) treatment on gene expression of mouse CD8+ T cells

Project description:To investigate the TVA's effect on mouse CD8+ T cells in vitro, we treated activated mouse CD8+ T cells with DMSO or 20 µM TVA for 24 hours. We then performed gene expression profiling analysis using data obtained from RNA-seq.

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Effect of trans-vaccenic acid(TVA) treatment on gene expression of mouse CD8+ T cells

Project description:To investigate the TVA's effect on mouse CD8+ T cells in vitro, we treated activated mouse CD8+ T cells with 20 µM TVA for various timepoints (or untreated cell control) followed by kethoxal labeling of single stranded DNA (ssDNA) We then performed differential analysis of ssDNA levels following the KAS-pipe pipeline

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