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Effect of metformin on mRNA expression, histone modifications, and chromatin accessibility in lung cancer cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE141060 | GEO | 2019/11/27

REPOSITORIES: GEO

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Effect of metformin on mRNA expression, histone modifications, and chromatin accessibility in lung cancer cells

Project description:Effect of metformin on mRNA expression, histone modifications, and chromatin accessibility in lung cancer cells

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Effect of metformin on histone modifications in lung cancer cells

Project description:The DNA regions that were associated with the metformin altered expressions of H3K4me3 and/or H3K27me3 were investigated by ChIP-seq, and the regions significantly up- or down-regulated by metformin were identified in H1299 cells. Over 80% of H3K4me3 were discovered within promoter regions, but less than 10% of H3K27me3 were observed in this area. Metformin significantly downregulated H3K4me3 level at the promoter regions of 1113 genes, but upregulated levels in the promoter regions of 1492 genes. Gene enrichment analysis revealed that abundant cell cycle related genes such as PIK3C3, CCNB2, CDK1, CDK6, and E2F8 were upregulated, while certain cell cycle suppressor genes such as CDKN1A (p21) and DDIT4 were downregulated. No specific biological functional cluster was detected in the H3K27me3 up- or downregulated genes.

2019-11-27 | GSE141053 | GEO

Effect of metformin on chromatin accessibility in lung cancer cells

Project description:The DNA regions whose chromatin accessibility was significantly altered by metformin were investigated in lung cancer cells by ATAC-seq. Metformin decreased chromatin accessibility in 159 regions but increased it in 124 sites. Most regions were outside of gene promoters defined as TSS ± 3kb. The most suppressed region was 4,667bp downstream of CHAC1, and the most enhanced site was 15,111bp downstream of ERRFI1.

2019-11-27 | GSE141059 | GEO