Optimized CRISPR-Cas12a platform for combinatorial drop-out genetic screening (RNA-seq)

ABSTRACT: Combinatorial genetic perturbations have been utilized to identify synthetic sick/lethal genetic interactions for cancer drug target discovery. Current methods for high-throughput combinatorial genetic screening are inefficient and cumbersome. Here we developed a simple, robust, and high-performance CRISPR-Cas12a-based approach for unbiased, combinatorial genetic screening in cancer cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE141130 | GEO | 2020/06/08

REPOSITORIES: GEO