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Role of splicing factor SRSF1 during the late stages of T cell maturation in the thymus

ABSTRACT: In thymus, the post-selection single positive thymocytes undergo further intrathymic maturation including down-regulation of CD69 and CD24, up-regulation of Qa-2, through which single positive thymocytes became equipped with emigration competency, then emigrate to periphery. Here we find that RNA binding protein Srsf1 is essential for the final maturation and survival of thymocytes.

ORGANISM(S): Mus musculus

PROVIDER: GSE141349 | GEO | 2021/04/21

REPOSITORIES: GEO

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Late stages of T-cell maturation in the thymus involve NF-ÎºB and tonic type I interferon signaling

Project description:Positive selection occurs in the thymic cortex, but critical maturation events occur later in the medulla. We defined the precise stage at which T cells acquire competence to proliferate and emigrate. Transcriptome analysis of late gene changes suggested roles for NF-ÎºB and interferon signaling. Mice lacking the IKK kinase TAK1, showed normal positive selection, but a specific block in functional maturation. NF-ÎºB signaling provided protection from TNF, and was required for proliferation and emigration. Alternatively, the interferon signature was independent of NF-ÎºB, and IFNÎ±R deficient thymocytes showed reduced STAT1 levels and phenotypic abnormality, but were competent to proliferate. Thus, both NF-ÎºB and tonic IFN signals are involved in the final maturation of thymocytes into naÃ¯ve T cells. Gene expression profile was performed on cells isolated from mouse thymus, including Pre-DP, Post-DP, SM&M1, M2 and Tak1-deficient SM&M1 cells.

2016-04-03 | E-GEOD-74078 | biostudies-arrayexpress

Late stages of T-cell maturation in the thymus involve NF-κB and tonic type I interferon signaling

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Role of AhR in thymocyte emigration in vivo

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Role of AhR in thymocyte emigration in vivo

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2021-05-25 | GSE163025 | GEO

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2012-01-01 | E-GEOD-31520 | biostudies-arrayexpress

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