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VELCRO-IP RNA-seq reveals ribosome expansion segment function in translation genome-wide

ABSTRACT: Roles for ribosomal RNA (rRNA) in gene regulation remain largely unexplored. With hundreds of rDNA units positioned across multiple loci, it is not possible to genetically modify rRNA in mammalian cells, hindering understanding of ribosome function. It remains elusive whether expansion segments (ESs), tentacle-like rRNA extensions that vary in sequence and size across eukaryotic evolution, may have functional roles in translation control. Here, we develop VELCRO-IP RNA-seq: a versatile methodology to generate species-adapted ESs and to map specific mRNA regions across the transcriptome that preferentially associate with ESs. By applying VELCRO-IP RNA-seq to a mammalian ES, ES9S, a large array of transcripts was identified that are selectively recruited to ribosomes via an ES. We further characterize a set of 5’ UTRs that facilitate cap-independent translation through ES9S-mediated ribosome binding. Thus, we present a technology for studying the enigmatic ESs of the ribosome, revealing their function in gene-specific translation.

ORGANISM(S): Mus musculus

PROVIDER: GSE141382 | GEO | 2021/01/29

REPOSITORIES: GEO

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