Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis between primary and iPS-derived monocytes and macrophages and comparison of iPS-derived macrophages between CCR5 patients and healthy controls

ABSTRACT: iPS-derived monocytes and macrophages are similar with primary monocytes and macrophages compared to iPS cells from the genome-wide overview and have similar gene expression patterns.

ORGANISM(S): Homo sapiens

PROVIDER: GSE141410 | GEO | 2019/12/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome analysis between primary and iPS-derived monocytes and macrophages and comparison of iPS-derived macrophages between CCR5 patients and healthy controls

Project description:Transcriptome analysis between primary and iPS-derived monocytes and macrophages and comparison of iPS-derived macrophages between CCR5 patients and healthy controls

| PRJNA593302 | ENA

RNA-Seq of LPA derived macrophages

Project description:Monocytes and macrophages constitute important components of immune system. Monocytes differentiate into macrophages following stimulation with cytokines and/or microbial molecule. Macrophages play central role in the maintenance of tissue homeostasis, development and its restoration after injury, as well as the initiation and resolution of innate and adaptive immunity. Though macrophages were long considered to be derived from differentiation of bone marrow monocytes, recent studies have proved that tissue resident macrophages are derived from yolk sac macrophages, fetal liver macrophages, can self-replicate from local proliferation. However, during homeostatic adaptations, injury and inflammation macrophages of different phenotypes can be recruited from the monocyte reservoirs of blood, spleen and bone marrow. Our studies show that Lysophosphatidic acid (LPA), a small lipid molecule converts monocytes into macrophages. We report the gene expression profile of primary mouse bone marrow monocytes treated for 5 days with LPA with respect to control monocytes.

2017-02-08 | GSE94626 | GEO

Human iPSC-derived monocytes and macrophages

Project description:Human iPSC-derived monocytes and macrophages

| PRJNA693399 | ENA

Ips calligraphus

Project description:Ips calligraphus overview

| PRJNA806287 | ENA

Ips nitidus

Project description:Ips nitidus overview

| PRJNA780645 | ENA

Ips typographus

Project description:Ips typographus overview

| PRJNA191580 | ENA

Ips pini

Project description:Ips pini overview

| PRJNA88143 | ENA

Comparison of transcriptional response of induced pluripotent stem iPS cell derived and monocyte derived macrophages to bacterial lipopolysaccharide stimulation

Project description:In the first part of this project, we will differentiate IPS cells from 5 human donors into macrophages, and extract RNA from unstimulated and LPS stimulated macrophages to perform RNA sequencing. We will also extract RNA before and after stimulation in blood- derived macrophages from 5 additional, unrelated healthy samples. In the second part of the project, RNA-seq data will be analysed to compare LPS response of these two macrophage populations. In summary, we will perform 75bp PE RNA-seq on 20 samples (10 pre and post stimulus), on the HiSeq 2500 platform. Samples will be multiplexed at 5 samples / lane, so we will require 4 flow cells in total.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2016-05-17 | E-ERAD-192 | biostudies-arrayexpress

The immunomodulatory effects of periostin on monocyte-derived macrophages

Project description:To explore the effects of periostin on tumor-associated macrophagess, we first sorted CD14+ monocytes from peripheral blood mononuclear cells of healthy donors using magnetic-activated cell sorting and then induced their differentiation into macrophages by culture with M-CSF for 5 days. In the absence of IL-4 stimulation, these macrophages expressed CD163 and Arg1 (a marker of M2 macrophage activation) but not CD206, suggesting that they were similar to macrophages in the early stage of mycosis fungoides (low frequency of CD206+ cells and low IL-4 expression). We then stimulated these macrophages with or without 100 ng/ml of periostin for 6 hours and examined gene expression using a cDNA microarray. Periostin stimulated gene expression on monocyte-derived macrophages was measured at 6 hours after exposure to doses of 100 ng/ml periostin.

2015-07-14 | E-GEOD-70821 | biostudies-arrayexpress

Monocyte-derived Macrophages

Project description:Chronic liver diseases as non-alcoholic steatohepatitis (NASH)-induced cirrhosis are characterized by an increasing accumulation of stressed, damaged, or dying hepatocytes. Hepatocyte damage triggers the activation of resident immune cells, such as Kupffer cells (KC), as well as the recruitment of immune cells from the circulation towards areas of inflammation. After infiltration, monocytes differentiate into monocyte-derived macrophages (MoMF) which are functionally distinct from resident KC. We herein aim to compare in vitro signatures of polarized macrophages and activated hepatic stellate cells (HSC) with ex vivo derived disease signatures from human NASH. Secondly, we investigate the effects of the secretome of primary human monocytes, macrophages and NK cells on HSC activation, as in particular monocytes and macrophages have often been linked to liver fibrosis progression. IL-4 and IL-13 treatment induced TGF-β1 secretion by macrophages. However, the supernatant transfer did not induce HSC activation. Interestingly, PMA-activated macrophages showed a strong induction of the fibrosis response genes COL10A1 and CTGF, while supernatant of IL-4/IL-13 treated monocytes induced the upregulation of COL3A1 in HSC. Supernatant of PMA-activated NK cells had the strongest effect on COL10A1 induction in HSC, while IL-15 stimulated NK cells reduced the expression of COL1A1 and CTGF. These data indicate that other factors, aside the well-known cytokines and chemokines, are potentially stronger contributors to activation of HSCs and induction of a fibrotic response, indicating a more diverse and complex role of monocytes, macrophages and NK cells in liver fibrosis progression.

2024-03-20 | GSE228087 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data