Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Setd2 reprograns lipid metabolism for the resolution of inflammation II

ABSTRACT: Purpose: The goal of this study is to identify differential expression profiles between WT and Setd2-KO in BMDMs induced by LPS for a time-course stimulaiton. Methods: We profiled RNA-seq in bone marrow-derived macrophages stimulated with LPS for 0h, 12h, 24h. Results: We find significant up and down regulated genes in Setd2-KO BMDMs under LPS stimulation.

ORGANISM(S): Mus musculus

PROVIDER: GSE141474 | GEO | 2022/11/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Setd2 reprograns lipid metabolism for the resolution of inflammation

Project description:Purpose: The goal of this study is to identify dynamic expression profiles of epigenetic genes in BMDMs induced by LPS for a time-course stimulaiton. Methods: We profiled RNA-seq in bone marrow-derived macrophages stimulated with LPS for 0h, 4h, 8h, 12h, 24h. Results: Epigenetic genes showed distinct expression patterns under LPS stimulation in different times

2022-11-13 | GSE141473 | GEO

Brain transcriptome of Larimichthys crocea (Large yellow croaker) under hypoxia

Project description:Brain transcriptome at 0h, 1h, 3h, 6h, 12h, 24h, 48h after hypoxia stress

2015-04-17 | GSE57608 | GEO

Gene expression dynamics underlying cell fate emergence in 2D micropatterned human embryonic stem cell gastruloids

Project description:We applied BMP4 to circular micropattern cultures to differentiate human embryonic stem cells (H1 hESCs) into microcolonies termed ‘gastruloids’, comprising germ layer and extraembryonic cells. Time-course single cell RNA-sequencing was performed at 0h (immediately before BMP4 treatment), 12h, and 24h after BMP4 treatment. Combining these 0h, 12h, and 24h datasets with previously generated 44h datasets, we report transcriptomic dynamics over the course of BMP4-driven differentiation in micropatterned cultures.

2021-04-22 | GSE169074 | GEO

Quantitative Analysis of Wild Type(wt) and Setd2 knockout(ko) mESCs Transcriptomes [RNA-Seq]

Project description:Purpose: This study aimed at exploring the deregulated genes in setd2 knockout mESCs compared with wt, more particularly to find the mechanism controlled by setd2,which was required for endoderm differentiation. Methods: Setd2 wt and ko mESCs were generated by deep sequencing, using Illumina GAIIx. Using Avadis NGS (version:1.3) software to analyze the sequence reads that passed quality filter to acquire the expression level of all genes. qRT–PCR validation was performed usingSYBR Green assays. Results: Using an optimized data analysis workflow, we mapped about 80 million sequence reads per sample to the mouse genome (build mm9) and identified 17,827 transcripts in the sted2 wt and ko mESCs. About 2,516 genes were deregulated in setd2 ko mESCs, more than 10 genes were validated using qRT-PCR. Conclusions: Through RNA-seq,we noticed that a subset of genes that related to MAPK signaling pathways were down-regulated in ko mESCs. This provided a bridge to connect setd2 and mESCs endoderm differentiation. One wt and one ko mESCs were generated by deep sequencing, using Illumina GAIIx.

2014-10-14 | E-GEOD-54932 | biostudies-arrayexpress

Stimulation of dendritic cells with LPS and R848

Project description:Samples of monocyte derived dendritic cells (moDCs) from three healthy volunteers untreated and treated with LPS [100ng/ml] or R848 [2.5µg/ml] for different time points (0h,3h,6h,12h and 24h).

2010-12-03 | E-MTAB-448 | biostudies-arrayexpress

Gene expression from bone-marrow derived macrophages.

Project description:Gene expression from WT and NFAT5 KO primary macrophage cultures. Keywords: Bone-marrow derived macrophages. We analyzed 4 arrays from each condition: unstimulated WT BMDMs, LPS stimulated WT BMDMs, unstimulated KO BMDMs, LPS stimulated KO BMDMs.

2012-01-20 | E-GEOD-26343 | biostudies-arrayexpress

The effect of Crem absence on gene expression in mouse liver.

Project description:CREM (cAMP responsive element modulator) together with CREB and ATF-1 belong to the CREB family of transcriptional factors, that respond to cyclic AMP signaling and bind to cAMP responsive element (CRE) sites in promoters of selected genes. CREM can produce isoforms that have either activating or repressing functions, depending on the transcription of specific exons. In this dataset, we include the expression data obtained from wild-type (WT) and Crem knock-out (KO) mouse liver. Several comparisons were made. KO<WT at 0h, KO<WT at 12 h, 0h<12h in WT and 0h<12h in KO.

2014-05-25 | GSE29594 | GEO

Expression data of CD117+ splenocytes from C57BL/6 WT and RasGRP4 KO mice upon LPS stimulation

Project description:RasGRP4 is required for CD117+ DCs to optimally induce certain NK cell-dependent immune responses in vivo and in vitro in response to LPSRasGRP4 expressed in DCs played an important role on NK cell IFN-γ secretion . We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated or down-regulated genes between the two DCs from WT and RasGRP4 mice respectively upon LPS stimulation. CD117+splenocytes were extracted from WT and RasGRP4 KO mice spleens aged 9-12 weeks and plated in 6-well plates with 1ug/mL of LPS for 0h,6h,12h,24h and 48h repectively for RNA extraction and hybridization on Affymetrix microarrays. Time course.

2015-05-19 | E-GEOD-69011 | biostudies-arrayexpress

RNA-sequencing shows the changes on transcriptomes after deletion of Setd2 in VHL deficient mice.

Project description:To explore the possible role of SETD2 in renal fibrosis formation, we generated VHL-KO and VHL-KO; SETD2-KO mice with a transgenic Ksp1.3/Cre mouse line. To get an insight into the mechanism of how SETD2 deletion promotes renal fibrogenesis in the absence of VHL, we performed RNA-seq using the renal tubules isolated from VHL-KO and VHL -KO; SETD2-KO mice

2022-10-19 | GSE213922 | GEO

IRG1 Inflammasome Proteomics Experiments

Project description:BMDMs of indicated phenotypes were stimulated with LPS for 24h and analyzed or restimulated with LPS for additional 3h and analyzed. In some experiments 1mM itaconate was added at 4h of LPS first stimulation.

2021-09-09 | PXD020089 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data