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LFY is pioneer transcription factor [ChIP-seq]

ABSTRACT: This study identified LEAFY (LFY) as a pioneer transcription factor. We made use of 35S:LFY-GR, an inducible version of the LFY protein fused to the rat glucocorticoid hormone binding domain. In root explants, steroid activated LFY-GR triggers synchronous and abundant flower induction (PMID: 15225291). We combined LFY ChIP-seq, MNase-seq before and after LFY binding and time-course RNA-seq in 35S:LFY-GR root explants to characterize the role of LFY as a pioneer transcription factor.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE141704 | GEO | 2020/10/27

REPOSITORIES: GEO

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LFY is pioneer transcription factor [MNase-seq]

Project description:This study identified LEAFY (LFY) as a pioneer transcription factor. We made use of 35S:LFY-GR, an inducible version of the LFY protein fused to the rat glucocorticoid hormone binding domain. In root explants, steroid activated LFY-GR triggers synchronous and abundant flower induction (PMID: 15225291). We combined LFY ChIP-seq, MNase-seq before and after LFY binding and time-course RNA-seq in 35S:LFY-GR root explants to characterize the role of LFY as a pioneer transcription factor.

2020-12-15 | GSE141705 | GEO

LFY is pioneer transcription factor [RNA-seq]

2020-12-15 | GSE141703 | GEO

Identification of LEAFY targets during reproductive transition

Project description:Global analysis of gene expression in 9 day old LEAFY-GR, 35S::LFY or Landsberg erecta seedlings treated with the steroid dexamethasone and/or the protein synthesis inhibitor cycloheximide. Keywords: other

2003-12-18 | GSE911 | GEO

Transcription profiling of Arabidopsis thaliana LFY mutant plants transformed with different LFY orthologs to investigate evolutionary divergence of LFY function

Project description:The aim of this experiment is to test the ability of the ortholog of Arabidopsis LFY gene from flowering and non flowering species to complement an Arabidopsis LFY mutant. Plants expressing, in a homozygous lfy12 background, the open reading frame of LFY orthologs under the control of Arabidopsis LFY promoter were synchronously induced to flower by growing plants in short days for 30 days then shifting them to Long Day for an additional 8 days. Shoot apices were dissected at either d0 or d8 in long days. Two biological replicates were performed. The following genotypes were used: Col - wild type arabidopsis; reference strain LFY (lfy12; LFY::AthLFY) - Arabidopsis UNI (lfy12; LFY::UNI) - Pisum ALF (lfy12; LFY::ALF) - Petunia WelNDLY (lfy12; LFY::WelNDLY) - Welwitschia mirabilis CrLFY1 (lfy12; LFY::CrLFY1) - Ceratopteris richardii CrLFY2 (lfy12; LFY::CrLFY2) - Ceratopteris richardii lfy-2; weak lfy allele lfy-9; intermediate leafy allele lfy-12; strong leafy allele.

2009-07-23 | E-MEXP-2258 | biostudies-arrayexpress

Transcription profiling of Arabidopsis LEAFY mutant seedlings treated with dexamethasone and/or cycloheximide

2007-07-12 | E-GEOD-911 | biostudies-arrayexpress

LFY is pioneer transcription factor

Project description:LFY is pioneer transcription factor

| PRJNA594407 | ENA

LFY is pioneer transcription factor [MNase-seq]

Project description:LFY is pioneer transcription factor [MNase-seq]

| PRJNA594411 | ENA

LFY is pioneer transcription factor [ChIP-seq]

Project description:LFY is pioneer transcription factor [ChIP-seq]

| PRJNA594412 | ENA

LFY is pioneer transcription factor [RNA-seq]

Project description:LFY is pioneer transcription factor [RNA-seq]

| PRJNA594413 | ENA

LEAFY target genes reveal a link between flower development and biotic stimulus response (III)

Project description:The transition from vegetative growth to flower formation is critical for the survival of flowering plants. The plant-specific transcription factor LEAFY (LFY) has central, evolutionarily conserved roles in this process, both in the formation of the first flower and later in floral patterning. We performed genome-wide binding and expression studies to elucidate the molecular mechanisms by which LFY executes these roles. Our study reveals that LFY directs an intricate regulatory network in control of floral homeotic gene expression and, unexpectedly, controls the expression of genes regulating the response to external stimuli in Arabidopsis. We further show that LFY dampens responses to a bacterial MAMP (microbe-associated molecular pattern) and to pathogen challenge. Our findings suggest a molecular mechanism for the coordination of reproductive stage development and disease response programs in plants. Regulation of these distinct survival programs by a single transcription factor may ensure optimal allocation of plant resources for reproductive fitness. Expression array analysis used to identify genes differentially expressed upon LFY induction in 9-day-old shoot apices. Expression array analysis of 9-day-old 35S::LFY-GR dexamethasone-treated seedlings compared to 9-day-old WT dexamethasone-treated seedlings. Four biological replicate samples.

2011-05-11 | E-GEOD-28062 | biostudies-arrayexpress

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