Genomics

Dataset Information

0

RNA Seq of TFH cells in Th1 vaccine regimen


ABSTRACT: Generating durable humoral immunity through vaccination depends upon effective interaction of follicular helper T cells (Tfh) with germinal center (GC) B cells. Th1 polarization of Tfh cells is an important process shaping the success of Tfh-GC B cell interactions by influencing co-stimulatory and cytokine-dependent Tfh help to B cells. However, the question remains whether adjuvant-dependent modulation of Tfh cells enhances HIV-1 vaccine-induced anti-Envelope (Env) antibody responses. We investigated whether an HIV-1 vaccine platform designed to increase the number of Th1-polarized Tfh cells enhances the magnitude and quality of anti-Env antibodies. Utilizing a novel interferon-induced protein (IP)-10-adjuvanted HIV-1 DNA prime, followed by an MPLA+QS-21-adjuvanted Env protein boost in macaques (DIP-10 PALFQ), we observed higher anti-Env serum IgG titers with greater cross-clade reactivity, specificity to V1V2, and effector functions when compared to macaques primed with DNA lacking IP-10 and boosted with MPLA+alum-adjuvanted Env protein (DPALFA) The DIP-10 PALFQ vaccine regimen elicited higher anti-Env IgG1 and lower IgG4 antibodies in serum, showing for the first time that adjuvants can dramatically impact the IgG subclass profile in macaques. The DIP-10 PALFQ regimen also increased vaginal and rectal IgA antibodies to a greater extent. Within lymph nodes, we observed augmented GC B cell responses and promotion of Th1 gene expression profiles in GC Tfh cells. The frequency of GC Tfh cells correlated with both the magnitude and avidity of anti-Env serum IgG. Together, these data suggest that adjuvant-induced stimulation of Th1-Tfh cells is an effective strategy for enhancing the magnitude and quality of anti-Env antibody response.

ORGANISM(S): Macaca mulatta

PROVIDER: GSE141707 | GEO | 2019/12/10

REPOSITORIES: GEO

Similar Datasets

2023-06-16 | GSE234813 | GEO
2022-07-21 | GSE179513 | GEO
| E-GEOD-81382 | biostudies-arrayexpress
2022-07-08 | GSE201551 | GEO
2016-09-07 | GSE81382 | GEO
| E-GEOD-32864 | biostudies-arrayexpress
2020-07-23 | GSE154897 | GEO
2016-12-31 | GSE89148 | GEO
| E-GEOD-69756 | biostudies-arrayexpress
2023-07-27 | GSE219118 | GEO