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ChIP-seq and R-ChIP-seq following BET bromodomain protein loss in HeLa cells

ABSTRACT: We report that treatment of HeLa cells with 2 hours of 100nM dBET6, which degrades BET bromodomain proteins, leads to global increases of RNA:DNA hybrids (R-loops) and DNA damage (γH2AX). R-loop occupancy was determined using a mutant version of RNase H1 (D210N) which binds to, but cannot resolve, R-loops.

ORGANISM(S): Homo sapiens

PROVIDER: GSE141763 | GEO | 2019/12/11

REPOSITORIES: GEO

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