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Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Brd4

ABSTRACT: We transfected C2C12 cells with 100nM of scrambled siRNA scramble and an siRNA directed against Brd4. Cells were harvested at 48h post-transfection and RNAseq was performed with polyA selection

ORGANISM(S): Mus musculus

PROVIDER: GSE141777 | GEO | 2022/09/28

REPOSITORIES: GEO

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CUT-tag analysis of T24 cells treated with a control scramble (scr) siRNA or a si-circSLC38A1

Project description:This study aims to investigate whether circSLC38A1 could affect the enrichment of downstream genes by the transcription factor ILF3.T24 cells were treated with a control scramble (scr) siRNA or a siRNA that specifically knock down the expression of circSLC38A1, 48h after ttansfection, cells were collected and treated for CUT-tag.CUT-tag-Seq analysis provided evidence of ILF3 enrichment of downstream genes changes between si-NC and si-circSLC38A1 T24 cells.

2022-10-01 | GSE186225 | GEO

PRC SiRNA vs scramble transfection in XTC.UC1 cells

Project description:PGC-1 related coactivator (PRC) shares structural and functional features with PGC-1{alpha}. It regulates several metabolic pathways and mitochondrial biogenesis. Its specific role in the early programming of cell proliferation suggests a finely regulated crosstalk between the mitochondrial functions and the cell cycle status. To explore the PRC-regulated pathways, we used a cell-line model of mitochondrial-rich tumors, presenting an oxidative metabolism and a specific increase in PRC expression. Microarray studies of temporal PRC invalidation in these XTC.UC1 cells were compared to the functional status of the mitochondria as well as to the expression level of genes and proteins involved in the oxidative phosphorylation process. Compared with what was observed for PGC-1{alpha}, we explored the role of nitric oxide in the PRC-regulated mitochondrial biogenesis. We proved that nitric oxide rapidly influences the expression of PRC at the transcriptional level. Focusing on mitochondrial energy metabolism, we demonstrated that PRC differentially controls the respiratory chain complexes and the coupling efficiency, in order to conserve a sufficient level of ATP and to protect the cell from oxidative stress. Our results highlight the key role of the PRC coactivator in the fine modulation of metabolic functions in response to the cell cycle status. Keywords: Transcriptionnal coactivator invalidation by SiRNA Expression profiles at 0h, 12h, 24h and 48h of PRC SIRNA treatment compared to scramble and referred to time of 20% serum induction. Expression profiles of PRC SIRNA and scramble during serum starvation were referred as -1.

2010-05-14 | E-GEOD-14282 | biostudies-arrayexpress

miRNA expression profiles of PRC SiRNA vs scramble transfected XTC.UC1 cells

2011-11-22 | E-GEOD-33843 | biostudies-arrayexpress

Effect of H19 Knockdown by siRNA on gene expression in C2C12 cells

Project description:We used microarrays to characterize the global changes in gene expression in C2C12 cells due to siRNA knockdown of long non-coding RNA H19 Control siRNA or siRNA specific for mouse H19 were transfected into day1 differentiating C2C12 myoblasts in triplicates. 40 H later total RNAs were isolated and subjected with microarray analysis.

2013-09-30 | E-GEOD-49535 | biostudies-arrayexpress

Effect of H19 Knockdown by siRNA on gene expression in C2C12 cells

Project description:We used microarrays to characterize the global changes in gene expression in C2C12 cells due to siRNA knockdown of long non-coding RNA H19

2013-09-30 | GSE49535 | GEO

Pimary human keratinocyte knockdown with deltaNp63 specific siRNA

Project description:Human ΔNp63-specific siRNA was obtained from Invitrogen (Carlsbad, CA; sense: 5′-ACAAUGCCCAGACUCAAUU-3′; antisense: 5′-AAUUGAGUCUGGGCAUUGU-3′). Scrambled sequence siRNA for the negative control was purchased from Invitrogen. Transfections were performed using Lipofectamine RNAiMAX (Invitrogen) in Opti-MEM (GIBCO) at 40 nmol/L according to the manufacturer's instructions. The culture medium was replaced at 6 h after siRNA transfection. mRNA was extracted from NHEKs transfected with ΔNp63-specific or scramble sequence siRNA at 72 h after transfection. Microarray slides were scanned using a 3D-GENE human 25k (TORAY, Tokyo, Japan) and microarray images were automatically analyzed with AROSTM, version 4.0 (Operon Biotechnologies, Tokyo, Japan).

2020-12-08 | GSE162900 | GEO

siRNA profiling of mouse myoblast C2C12 cells reveals PPARbeta/delta dependent differentiation

Project description:siRNA profiling of mouse myoblast C2C12 cells reveals PPARbeta/delta dependent differentiation

2012-02-01 | E-MTAB-528 | biostudies-arrayexpress

Transcriptomic analysis after transfection in murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) of either a siRNA against SOX2 or a scramble siRNA

Project description:SOX2 is the main gene involved in anophthalmia. In order to identify genes regulated by SOX2 transcription factors (genes that could be good candidates to also be involved in ocular development), we studied transcriptomic profiles of murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) after transfection by a siRNA against SOX2 or a scramble siRNA. murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) after transfection by a siRNA against SOX2 or a scramble siRNA

2015-11-04 | E-GEOD-74598 | biostudies-arrayexpress

Transcriptomic analysis after transfection in murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) of either a siRNA against SOX2 or a scramble siRNA

2015-11-04 | GSE74598 | GEO

Identification of differentially expressed genes in Sfmbt1-knockdown C2C12 myoblasts

Project description:Gene expression profiling was performed to identify Sfmbt1-dependent regulation in myogenic programs. To establish the magnitude of the Sfmbt1 effect on muscle cells, we have compared gene expression profiles of C2C12 cells transduced with lentiviruses expressing scramble shRNA control or shSfmbt1. Our analysis suggested that Sfmbt1 critically confers transcriptional silencing of muscle genes in myogenic progenitor cells. Two biological replicates for each sample group: C2C12 cells stably transduced with pLKO.1 expressing scramble shRNA control and C2C12 cells transduced with pLKO.1 shSFMBT1. Samples were independently cultured and RNAs were then harvested for microarray analysis.

2013-01-28 | E-GEOD-24346 | biostudies-arrayexpress

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