Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signalling

ABSTRACT: Cell replacement therapies hold great therapeutic potential. Nevertheless, our knowledge of the mechanisms governing the developmental processes is limited, impeding the quality of differentiation protocols. Generating insulin-expressing cells in vitro is no exception, with the guided series of differentiation events producing heterogeneous cell populations that display mixed pancreatic islet phenotypes and immaturity. The achievement of terminal differentiation ultimately requires the in vivo transplantation of, usually, encapsulated cells. Here we show the impact of cell confinement on the pancreatic islet signature during the guided differentiation of alginate encapsulated human induced pluripotent stem cells (hiPSCs). Our results show that encapsulation improves differentiation by significantly reshaping the proteome landscape of the cells towards an islet-like signature. Pathway analysis is suggestive of integrins transducing the encapsulation effect into intracellular signalling cascades promoting differentiation. These analyses provide a molecular framework for understanding the confinement effects on hiPSCs differentiation while confirming its importance for this process.

ORGANISM(S): Homo sapiens

PROVIDER: GSE141891 | GEO | 2020/01/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Alginate encapsulation of differentiating hiPSCs

Project description:Here we characterize the impact of cell confinement on the pancreatic islet signature during the guided differentiation of alginate encapsulated human induced pluripotent stem cells.

2020-02-12 | PXD012704 | Pride

Transcriptomic Responses of Synechocystis sp. PCC 6803 Encapsulated in Silica Gel

Project description:Global gene expression of Synechocystis sp. PCC 6803 encapsulated in silica gel was examined by microarray analysis. Cultures were encapsulated in gels derived from aqueous precursors and gels derived from alkoxide precursors and incubated under constant light for 24 hours prior to RNA extraction. Cultures suspended in liquid media were also exposed to 500 mM salt stress and incubated under identical conditions, for comparison purposes. The expression of 414 genes was significantly altered by encapsulation in aqueous-derived gels (fold change >/= 1.5 and P-value < 0.01), the expression of 1143 genes were significantly altered by encapsulation in alkoxide derived gels, and only 243 genes were common to both encapsulation chemistries. Additional qRT-PCR analyses of four select genes; ggpS, cpcG2, slr5055, and sll5057, confirmed microarray results. These results illustrate that encapsulation stress is quite different than salt stress in terms of gene expression response. Furthermore, a number of hypothetical and unknown proteins associated with encapsulation and alcohol stress have been identified, with implications for improving encapsulation protocols and rationally engineering microorganisms for direct biofuel production. 16 samples; 4 biological replicates each of 4 treatments

2012-04-23 | E-GEOD-37482 | biostudies-arrayexpress

Dynamic proteome profiling of human induced pluripotent stem cell-derived pancreatic progenitors

Project description:Quantitative description of proteome dynamics during in vitro differentiation of hiPSCs towards pancreatic progenitor cells, providing time-specific protein signatures.

2021-08-30 | PXD003338 | Pride

Modulation of mitochondrial DNA copy number to induce hepatocytic differentiation of human Amniotic Epithelial cells

Project description:Human amniotic epithelial cells were differentiated into hepatocyte-like cellsfor 28 days and gene expression evaluated. Human amniotic epiethelial cells were encapsulated in barium alginate microcapsules and differentiated into hepatocyte-like cells to create 3D environment for differentiation and gene expression evaluated. The aim of the study was to evaluate changes in gene expression during hepatic diffrentiation and effects of encapsulation on differentiation.

2017-09-03 | GSE100688 | GEO

Transcriptomic Responses of Synechocystis sp. PCC 6803 Encapsulated in Silica Gel

2012-04-24 | GSE37482 | GEO

Differentially regulated genes on day 10 of pancreatic differentiation of hiPSCs

Project description:To determine the pancreatic genes which are upregulated after 10 days of pancreatic differentiation of hiPSCs

2016-11-10 | GSE60372 | GEO

High-efficiency cellular reprogramming with microfluidics

Project description:We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-β and other signaling pathways exhibited temporal regulation that appears critical to acquisition of pluripotency. The high quality and purity of the resulting hiPSCs (μ-hiPSCs) allowed direct differentiation into functional hepatocyte- and cardiomyocyte-like cells in the same platform without additional expansion.

2016-04-10 | GSE59534 | GEO

Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signalling

Project description:Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signalling

| PRJNA595104 | ENA

microRNA and mRNA expression profiles of human pancreatic islet-like cell clusters

Project description:Type 1 diabetes is an autoimmune destruction of pancreatic islet beta cell disease, and it is important to find new alternative source of the islet beta cells to replace the damaged cells. Human embryonic stem (hES) cells possess unlimited self-renewal and pluripotency and thus have the potential to provide an unlimited supply of different cell types for tissue replacement. The hES-T3 cells with normal female karyotype were first differentiated into embryoid bodies and then induced to generate the pancreatic islet-like cell clusters, which expressed pancreatic islet cell-specific markers of insulin, glucagon and somatostatin. The expression profiles of microRNAs and mRNAs from the pancreatic islet-like cell clusters were further analyzed and compared with those of undifferentiated hES-T3 cells and differentiated embryoid bodies. MicroRNAs negatively regulate the expression of protein-coding mRNAs. The pancreatic islet-like cell clusters were found to exhibit very high expression of microRNAs miR-186, miR-199a and miR-339, which down-regulated the expression of LIN28, PRDM1, CALB1, GCNT2, RBM47, PLEKHH1, RBPMS2 and PAK6. Therefore, these microRNAs are very likely to play important regulatory roles in the differentiation of pancreatic islet cells and early embryonic development. In this investigation, both miRNA and mRNA expression profiles from the pancreatic islet-like cell clusters differentiated from hES-T3 cells (T3pi) were quantitatively determined and compared with those of undifferentiated hES-T3 cells grown on mouse embryonic fibroblast (MEF) feeder (T3ES) and embryoid bodies differentiated from hES-T3 cells (T3EB). Several target genes of pancreatic islet cell-specific miRNAs were identified. ***This submission represents the mRNA expression component of the study only***

2010-09-29 | E-GEOD-14503 | biostudies-arrayexpress

Differentially regulated genes on days 0, 12 and 17 of pancreatic differentiation of control and MODY5 hiPSCs

Project description:To determine the differentially expressed genes between control and MODY5 after 0, 12 and 17 days of pancreatic differentiation of hiPSCs

2016-11-10 | GSE74884 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data