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Florigen family chromatin recruitment, competition and targets [RNA-Seq]

ABSTRACT: This study identified genes that re-upregulated in far-red enriched photoperiod in the wild type or tfl1-1, but not in ft-10 (FT-dependent photoinduction). We performed RNA seq on tfl1-1, wild type and ft-10 grown for 42 days in short-day conditions and treated for 24 hours with far-red enriched photoinduction (24 hr FRP) or grown for 43 days in short-day conditions (no FRP). RNA was isolated from trimmed and dissected non-bolted inflorescences.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE141893 | GEO | 2020/09/01

REPOSITORIES: GEO

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Florigen family chromatin recruitment, competition and targets [ChIP-Seq]

Project description:This study identified direct targets of TFL1 and FD and showed that TFL1 is recruited to the chromatin by FD. We performed ChIP-seq using anti-GFP antibodies in gTFL1-GFP tfl1-1, gTFL1-GFP fd-1 and controls (nontransgenic plants). We used anti-GUS antibodies for gFD-GUS fd-1. ChIP was conducted on dissected, non-bolted, inflorescences from 42-day-old short-day-grown Arabidopsis thaliana.

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Transcriptomic analysis of the interaction between FLOWERING LOCUS T induction and photoperiodic signaling in response to spaceflight

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SIN3 LIKE genes mediate long-day induction of flowering but inhibit the floral transition in short days through histone deacetylation in Arabidopsis

Project description:We show that in Arabidopsis SIN3 LIKE (SNL)family genes encoding a scoffold protein for assembly of histone deacetylase complex, directly regulate the expression of an FT activator and three FT repressors to regulate the transition to flowering in short days and long days, respectively. Under inductive long days, SNLs including SIN3 LIKE 1(SNL1) to SNL5, function in partial redundancy to repress the expression of three AP2 family transcription factors that repress FT expression, and thus mediate long-day induction of FT expression and promote the transitiion to flowering. In contrast, under non-inductive short days SNLs act to inhibit the floral transition, partly through direct repression of a MADS box transcriptional factor that promotes FT expression. Thus, our results reveal that SNLs, through histone deacetylation, play a novel dual role for the control of flowering in the long-day plant Arabidopsis: inhibiting flowering when the day length is shorter and promoting the floral transition when days become longer than a threshold length.

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Molecular basis of flowering under natural long-day conditions in Arabidopsis

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Arabidopsis thaliana analysis of the shade avoidance response in Col-0, rev5, and wox4 mutants

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2013-03-01 | E-GEOD-38989 | biostudies-arrayexpress

Transcriptome analysis of the effect of far-red light on hypocotyl elongation of pumpkin

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