Profiling Kif5A RNA targets in sensory neuron axons

ABSTRACT: We previously identified that the RNA binding protein Nucleolin is localized to axons of DRG sensory neurons by interaction with the molecular motor Kinesin-1 (Kif5A) and subsequently localizes importin beta1 mRNA there (Perry et al., 2016). To further identify additional RNAs that are localized to axons in a similar mechanism, we immunopercipitated Kif5A from dorsal roots (centrally projecting axons) or Sciatic nerves (peripherally projecting axons), isolated the bound RNA and sequnced it.

ORGANISM(S): Mus musculus

PROVIDER: GSE142099 | GEO | 2021/07/23

REPOSITORIES: GEO