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Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC, also known as ASC)

ABSTRACT: The effect of standard tissue culture on AdMSC is not well defined, therefore we sought to analyse global changes in microRNA expression when a defined ex vivo population of AdMSC was subjected to standard tissue culture conditions.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE142429 | GEO | 2019/12/21

REPOSITORIES: GEO

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Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC)

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Project description:Next Generation Sequencing (NGS)-based mRNA sequencing (RNA-seq) has provided high-throughput measurements to analyze the transcriptome of cells. We are reporting comprehensive transcriptome profiling of primarily isolated human adipose-derived mesenchymal stem cells (ADMSC) throughout consecutive culture passages using NGS-based RNA-seq method. Human adipose tissue was obtained from operative remains and and digested in collagenase X. ADMSC were isolated, cultivated and characterized as mesenchymal stem cells (MSC) according to the minimum criteria for defining multipotent MSC from the International Society for Cellular Therapy (ISCT). mRNA profiles of ADMSC in p0, p1 and p2 were quality controlled, and generated by deep sequencing, in triplicate, using Illumina PE150 kits. Paired-end raw data were pre-processed using a published protocol. HISAT2 (Hierarchical Indexing for Spliced Alignment of Transcripts) was used to align reference genome. HTSeq was used to calculate Reads Count, and gene abundence was determined by analyzing FPKM (fragments per kilobase of exon model per million reads mapped).

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