Defining the influence of the A12 subunit on transcription elongation and termination by rna polymerase I

ABSTRACT: we performed Native Elongating Transcript Sequencing (NET-seq) on an rpa12Δ strain of S. cerevisiae and evaluated the resultant change in Pol I occupancy throughout the 35S gene and the IGS. Compared to WT, we observed template sequence-specific changes in Pol I occupancy throughout the 35S gene. We also observed rpa12Δ Pol I occupancy downstream of both termination sites and throughout most of the IGS, including the 5S gene. Occupancy of rpa12Δ Pol I increased just upstream of the promoter proximal Reb1 binding site and dropped significantly after, implicating this site as a third terminator for Pol I transcription.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE142457 | GEO | 2019/12/21

REPOSITORIES: GEO