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GAR-Driven Subcellular Localization of the Growth Controlling RNA Binding Protein Nucleolin

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE142576 | GEO | 2021/07/23

REPOSITORIES: GEO

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GAR-Driven Subcellular Localization of the Growth Controlling RNA Binding Protein Nucleolin

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Project description:In this project we aimed to identify the spectrum of protein binders of nucleolin’s glycine arginine reach (GAR) domain. We did so by designing a 15 amino acid peptide derived from the GAR domain sequence, which includes 3 arginine-glycine-glycine (RGG) repeats and we call "R3". We conducted affinity purification with biotinylated R3 peptide, from mouse sciatic nerve axoplasm preparations, followed by mass spectrometry, in order to identify potential GAR interacting proteins.

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