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Expression data from Il-27 stimulated normal human epidermal keratinocytes (NHEK) and THP-1 cells

ABSTRACT: IL-27 plays a pivotal role in regulating complex immune responses. To evaluate the role of IL-27 in modulation in the expression of cellular and innate immunity-asscoiated genes in human epidermal keratinocytes (primary cells) and the monocytic THP-1 cell line (pre-treated with PMA) were stimulated with IL-27 and genome-wide expression of these cells were investigated utilizing microarray assay.

ORGANISM(S): Homo sapiens

PROVIDER: GSE143228 | GEO | 2020/01/08

REPOSITORIES: GEO

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Expression data from Il-27 stimulated normal human epidermal keratinocytes (NHEK) and THP-1 cells

Project description:Expression data from Il-27 stimulated normal human epidermal keratinocytes (NHEK) and THP-1 cells

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A combinatorial atlas of RNA Polymerase III subunit occupancy in human THP-1 cells

Project description:We present a genome-wide map of RNA Polymerase III subunit localization in human THP-1 monocytes and THP-1 derived macrophages after 72 hr exposure to PMA, as well as profiles of POLR3G and POLR3GL occupancy in THP-1 monocytes after 4 hr exposure to Pol III drug inhibitor ML-60218; 27 uM

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RNA Sequencing of PGE2 or MEHP treated human THP-1 derived macrophages transcriptomes

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Transcription and chromatin profiling in differentiating THP-1 monocytes and THP-1 derived macrophages

Project description:Here we profile nascent transcription, RNA polymerase III occupancy, chromatin accessibility, and H3K27ac levels in THP-1 monocytes and THP-1 derived macrophages after 72 hr exposure to phorbol myristate acetate (PMA).

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Transcriptomics of THP-1 macrophages treated with or without IL-26 for 24h

Project description:Purpose: The aim of this study was to obtain a comprehensive transcriptomic analysis of IL-26-exposed macrophages. Methods: THP-1 derived macrophage were generated by incubating THP-1 cells with PMA (50 ng/ml) for 24h.Then THP-1 macrophages were treated with IL-26 for 24h, which were collected and applied to RNA-sequencing. The constructed sequencing library was sequenced using an Illumina Novaseq 6000 system. The expression of transcripts was calculated as fragments per kilobase of exon model per million mapped reads (FPKM). Results: In total, we identified 1303 differentially expressed protein-coding genes between untreated and IL-26-treated macrophages, including 667 up-regulated and 636 down-regulated genes.

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Differentiation of cultured THP-1 cells

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2004-11-19 | GSE1439 | GEO

Differentiation of cultured THP-1 cells

Project description:Libraries constructed from cultured THP-1 cells receiving PMA treatment or not. Keywords: other

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