Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome changes in mouse retina after conditional knockout of UXT

ABSTRACT: Ubiquitously expressed transcript (UXT), a small chaperone-like protein, is widely expressed in diverse human and mouse tissues and is more abundant in retina and kidney. Here, we investigated changes after conditional knockout of UXT in mouse retina at molecular, morphological and functional aspects.

ORGANISM(S): Mus musculus

PROVIDER: GSE143281 | GEO | 2020/08/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome changes after overexpression, knockdown and condItional knockout of UXT in mice

Project description:Ubiquitously expressed transcript (UXT) has been shown to play a role in regulating the process of autophagy. Here, we investigated roles of UXT in the innnate immune responses after overexpression, knockdown and condItional knockout of UXT in mice

2024-05-18 | GSE175928 | GEO

Transcriptome changes in mouse retina after conditional knockout of UXT

Project description:Transcriptome changes in mouse retina after conditional knockout of UXT

| PRJNA599586 | ENA

UXT is required for spermatogenesis in mice

Project description:Male mammals must simultaneously produce prodigious numbers of sperm and maintain an adequate reserve of stem cells to ensure continuous production of gametes throughout life. Failures in the mechanisms responsible for balancing germ cell differentiation and spermatogonial stem cell (SSC) self-renewal can result in infertility. We discovered a novel requirement for Ubiquitous Expressed Transcript (UXT) in spermatogenesis by developing the first knockout mouse model for this gene. Constitutive deletion of Uxt is embryonic lethal, while conditional knockout in the male germline results in a Sertoli cell-only phenotype during the first wave of spermatogenesis that does not recover in the adult. This phenotype begins to manifest between 6 and 7 days post-partum, just before meiotic entry. Gene expression analysis revealed that Uxt deletion downregulates the transcription of genes governing SSC self-renewal, differentiation, and meiosis, consistent with its previously defined role as a transcriptional co-factor. Our study has revealed the first in vivo function for UXT in the mammalian germline as a regulator of distinct transcriptional programs in SSCs and differentiating spermatogonia.

2018-04-18 | GSE111740 | GEO

EZH1/SUZ12 complex positively regulates the transcription of NF-κB target genes via interaction with UXT

Project description:EZH1 is one of polycomb group genes which positively regulates transcription in the genome-wide scale. However, the underlying mechanisms still remain elusive. Here we report that EZH1 physically interacts with UXT, one small chaperon-like transcription co-activator of NF-κB. UXT specifically interacts with EZH1 and SUZ12, but not EED or EZH2. Similar to UXT, RNA interference of EZH1 and SUZ12 in the cell impairs the transcriptional activation of NF-κB target genes after TNFα treatment, and increases the induced cell death. Then RNA-Sequencing analysis was used to analysis the transcriptome in HCT116 cells after EZH1 or UXT knockdown. EZH1 or UXT deficiency nearly impaired the induced expression of all the genes by TNFα. ChIP-Sequencing was used to analysis the H3K27me1, H3K27me2 and H3K27me3 levels in HCT116 cells after EZH1 or UXT knockdown. However, the results show that H3K27 mono-, di- and trimethylation on NF-κB target genes are not affected with EZH1 or UXT deficiency. EZH1 does not affect the translocation of RELA/p65 from cytosol to nuclear either. Instead, EZH1 regulates the recruitment of RELA/p65 and RNA POLII to target genes. Taken together, our study demonstrates EZH1 as a positive regulator for NF-κB signaling and reveals the underlying mechanism how EZH1 and UXT work together in transcription regulation.

2016-05-11 | GSE75217 | GEO

RNA-Seq of UXT KO in Primary Sertoli cells

Project description:We created a knockout model of ART-27(UXT) specially in the Sertoli cells of the mouse testes. In order to see how loss of ART-27 effects the Sertoli cell transcriptome we isolated Sertoli cells from WT and KO animals. The WT cells were infected with a vector only construct, and the KO cells were infected with CRE producing construct.

2020-07-31 | GSE155412 | GEO

The Comprehensive Mouse Retina Proteome

Project description:Diverse groups of proteins play integral roles in both the physiology and pathophysiology of the mouse retina. A comprehensive mouse retina proteome has been developed using a discovery-based proteomic approach.

2019-02-16 | PXD009909 | Pride

The Comprehensive Mouse Retina PhosphoProteome

Project description:Diverse groups of proteins play integral roles in both the physiology and pathophysiology of the mouse retina. A comprehensive mouse retina phosphoproteome has been developed using a discovery-based proteomic approach in conjunction with an enrichment procedure for phosphopeptides.

2019-02-16 | PXD009981 | Pride

Gene expression differences between wildtype and Atrx conditional knockout mouse retina tissues

Project description:Loss of the Atrx chromatin remodeling protein causes dysfunction and death of post-mitotic retinal interneurons in mice. Embryonic conditional deletion of Atrx from multipotent retinal progenitor cells results in the selective loss of the retinal inhibitory interneurons, namely amacrine and horizontal cells. The cell death occurs postnatally after the development of these cell types, peaking at postntal day 17 in the mouse retina. Identification of molecular factors and pathways that mediate the health and survival of these neurons may suggest novel therapeutic strategies for neuroprotection in ATR-X syndrome and other neurodegenerative diseases. We performed gene expression profiling of wildtype and Atrx conditional knockout mouse retina tissues to identify putative targets of Atrx and molecular pathways that underlie the neurodegenerative phenotype.

2016-09-09 | GSE78877 | GEO

UXT is required for spermatogenesis in mice

Project description:UXT is required for spermatogenesis in mice

| PRJNA438005 | ENA

mouse retina 10x Genomics Multiome ATAC+RNA data

Project description:Mouse retina is heterogeneous, composed of multiple neuronal and non-neuronal cell types. Among them, bipolar cells (BC), which connect photoreceptors (cones and rods) to inner retina, are traditionally dissected into rare subtypes of subtle functional and morphological differences. While high-resolution single-cell transcriptomic profiles of BCs are availabl, little is known about the corresponding single-cell chromatin landscapes. Although it is now possible to directly generate multiome data, there are often restrictions on cost, feasibility, and data quality. Therefore, integrating single-cell ATAC and RNA profiles obtained independently from the same retina sample may provide an exciting opportunity to comprehensively characterize these rare cell subtypes and discover transcription factors (TFs) important in establishing or maintaining the cell identities

2022-05-02 | GSE201402 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data