Global RNA stability profiling reveals stabilization of P-body-enriched transcripts in the absence of human Dcp2

ABSTRACT: Multiple RNA decapping enzymes coexist in mammalian cells to regulate decay of distinct subsets of cellular transcripts, but their specificity remains incompletely defined to date. Here we present a global profile of RNA stability changes in human Dcp2 knockout cells via TimeLapse-seq. We demonstrate that P-body enrichment is the strongest correlate of Dcp2-dependent RNA decay, and that post-transcriptional modifications such as m6A present additive effect for Dcp2 targeting. Importantly, our data support a model in which P-bodies are sites that sort translationally repressed transcripts for cytoplasmic decay through additional molecular marks.

ORGANISM(S): Homo sapiens

PROVIDER: GSE143662 | GEO | 2020/05/06

REPOSITORIES: GEO