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Epithelial planar bipolarity emerges from Notch- mediated asymmetric inhibition of Emx2

ABSTRACT: This dataset consists of single-cell RNA-seq (mcSCRBseq) data of neuromast hair cells from wild type and Emx2 mutant zebrafish larvae. Posterior lateral-line neuromast hair cells were isolated by fluorescence activated cell sorting (FACS) from the dissociated trunks of wild type and Emx2-mutant Tg[myo6b:actb1-EGFP] transgenic zebrafish larvae expressing the green fluorescent protein EGFP in hair cells and unipotent hair-cell progenitors (UHCPs).

ORGANISM(S): Danio rerio

PROVIDER: GSE143663 | GEO | 2020/03/05

REPOSITORIES: GEO

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Epithelial planar bipolarity emerges from Notch-mediated asymmetric inhibition of Emx2

Project description:This dataset consists of single-cell RNA-seq (10X) data of neuromast cells from zebrafish larvae. Posterior lateral-line neuromast cells were isolated by fluorescence activated cell sorting (FACS) from the dissociated trunks of Tg[myo6b:actb1-EGFP] transgenic zebrafish larvae expressing the green fluorescent protein (EGFP) in hair cells and unipotent hair-cell progenitors (UHCPs), and from Tg[Et(krt4:EGFP)sqgw57A] larvae expressing EGFP in supporting cells.

2020-02-06 | GSE144827 | GEO

Emx2 defines bidirectional polarity of neuromasts by changing hair-bundle orientation and not hair-cell positions

Project description:The bidirectional sensitivity of neuromasts to water flow in the zebrafish lateral line system is attributed to the opposite orientation of the hair bundles on top of sensory hair cells (HC) within a neuromast. After each HC precursor divides to form two nascent HCs, HCs of the same bundle orientation are positioned on one side of the neuromast, across from HCs with opposite bundle orientation. The transcription factor emx2 is expressed in only one of the sibling HCs. Loss or gain of function of emx2 in HCs causes unidirectional hair bundle orientation in neuromasts. It is not clear whether Emx2 is required specifically in establishing hair bundle orientation after HC formation or Emx2 has an earlier role in HC fate and/or positioning, which indirectly changes hair bundle orientation. A phenomenon, in which nascent sibling HCs exchange positions with each other, has been postulated to be the mechanism for HCs to acquire their designated positions within the neuromast. We asked whether Emx2 is involved in positional acquisition of HCs within the neuromast. Using live-imaging and our emx2 reporter we find that the HC rearrangement, redefined as two processes named Rock & Roll are required for HCs to acquire their positions. Although Emx2 regulates the duration of the Rock and the frequency of Roll of nascent HCs, it is not required for their positional acquisition. Instead, Emx2 regulates the morphology of nascent HCs, which facilitates the rearrangement process.

2021-01-19 | GSE152859 | GEO

Epithelial planar bipolarity emerges from Notch- mediated asymmetric inhibition of Emx2

Project description:Epithelial planar bipolarity emerges from Notch- mediated asymmetric inhibition of Emx2

| PRJNA601204 | ENA

Epithelial planar bipolarity emerges from Notch-mediated asymmetric inhibition of Emx2

Project description:Epithelial planar bipolarity emerges from Notch-mediated asymmetric inhibition of Emx2

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Expression data from Emx2 wildtype and knockout olfactory epithelium

Project description:Emx2 is a homeobox transcription factor that plays a critical role in development. Olfactory sensory neuron axons from Emx2 knockout mice fail to innervate their target tissue, the olfactory bulb. Homeobox transcription factors may also play an important role in olfactory receptor expression. We used microarrays to analyze differences in mRNA abundance in the olfactory epithelium of Emx2 wildtype and knockout embryonic day 18.5 mice. Keywords: Knockout-Wildtype comparision Total RNA from 9 Emx2 wildtype and 9 Emx2 knockout mice was analyzed. Equal amounts of RNA from 3 animals was pooled to create one biological sample, 3 pools per genotype, 6 chips total. RNA was hybridized to the Affymetrix Mouse Exon 1.0 ST array.

2008-10-15 | E-GEOD-12135 | biostudies-arrayexpress

Gene expression analysis of hair cell regeneration in the zebrafish lateral line

Project description:Deafness due to the terminal loss of inner ear hair cells is one of the most common sensory diseases. However, non-mammalian animals (e.g. birds, amphibian and fish) regenerate damaged hair cells. In order to better understand the reasons underpinning such regeneration disparities in vertebrates, we set out to define the changes in gene expression associated with the regeneration of hair cells in the zebrafish lateral line at high resolution. We performed RNA-Seq analyses on regenerating support cells purified by fluorescence activated cell sorting (FACS). The zebrafish lateral line provides an experimentally accessible system to define the complex signaling events triggered by injury and regeneration, because these cells can be acutely killed by exposure to neomycin, after which they regenerate rapidly. Lateral line hair cells are located in the center of a mechanosensory organ known as the neuromast and are surrounded by inner support cells and an outer ring of mantle cells. Tg(sqET20) larvae express GFP strongly in mantle cells and to a lesser degree in inner support cells. We isolated GFP positive and GFP negative cells from 5 days post fertilization (dpf) Tg(sqET20) larvae at 1, 3 and 5 hours post neomycin treatment, as well as from a non-treated control. Transgenic zebrafish Tg(sqET20) larvae at 5 days post fertilization were exposed to neomycin, dissociated, and FACS sorted into GFP positive and GFP negative populations at 1, 3, and 5 hours following treatment, along with a mock treated 1 hr control. The experiment was performed in triplicate, for a total of 24 samples.

2014-03-26 | E-GEOD-56176 | biostudies-arrayexpress

Transcription profiling of mouse Emx2 knock-out urogenital epithelium

Project description:Series of samples studying effect of knock out Emx2 in urogenital epithelium of mouse embryos at E10.5. Experiment Overall Design: The epithelial cells of the gonadal primordium were obtained by Laser Microdissection System. The specimens prepared from three individuals were mixed as one pool. There are three experimental replicates in each genotype, 3-pools of wild-type and 3-pools of Emx2 KO mouse.

2008-06-11 | E-GEOD-10216 | biostudies-arrayexpress

Expression data from Emx2 wildtype and knockout olfactory epithelium

2008-10-15 | GSE12135 | GEO

Emx2 knock-out urogenital epithelium

Project description:Series of samples studying effect of knock out Emx2 in urogenital epithelium of mouse embryos at E10.5. Keywords: repeated sample

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Gene expression analysis of hair cell regeneration in the zebrafish lateral line

2014-03-26 | GSE56176 | GEO

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