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Knockout of the Caspase 8 Associated Protein 2 (CASP8AP2) gene in HEK293 cells leads to improvement in recombinant protein expression and cell cycle arrest at the G0/G1 phase

ABSTRACT: In this study, we focused on understanding the effect of the functional inactivation of the caspase 8 associated protein 2 (CASP8AP2) gene on gene expression in HEK293 cells, to possibly explain the improvement in recombinant protein expression engendered by its inactivation. To achieve this goal, the HEK293 cell line constitutively expressing luciferase was treated with CRISPR constructs targeting the CASP8AP2 gene, single clones isolated and a clone with a functionally compromised CASP8AP2 gene, 892-7, selected for further investigations. Recombinant luciferase expression, recombinant secreted alkaline phosphatase expression, growth and metabolic characteristics were evaluated and compared between the 892-7 clone and the parental cell line. Cells cultured to a confluence of about 80% were used for gene expression profiling. We found that genes involved in cell cycle progression and in histone mRNA synthesis were highly differentially expressed between the two cell lines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE143808 | GEO | 2020/09/22

REPOSITORIES: GEO

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