MiRNA profiling in RAW264.7 cells treated without or with LPS and IFNγ

ABSTRACT: We have identified a number of miRNAs that are differentially expressed in LPS and IFNγ treated RAW264.7 cells, as compared to untreated cells. Microarray and quantitative real-time PCR (qRT-PCR) results showed that miR-103 decreased while the STAT1 expression increased substantially in RAW264.7 derived M1 macrophage, and there was a significant negative correlation between miR-103 and STAT1.Overexpression of miR-103 suppressed M1 polarization by inhibiting STAT1/IRF1 signaling pathway and vice versa in vitro. STAT1 is a direct target of of miR-103.

ORGANISM(S): Mus musculus

PROVIDER: GSE143845 | GEO | 2020/10/28

REPOSITORIES: GEO