Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole transcriptome RNAseq analysis of somatic cells in Inhba fetal testes

ABSTRACT: We performed RNA-seq on testicular somatic cells from fetal activin A-deficient mice (Inhba KO) and wildtype littermates at embryonic ages E13.5 and E15.5. Activin A deficiency predominantly affects the Sertoli cell transcriptome. New candidate targets include Minar1, Sel1l3, Vnn1, Sfrp4, Masp1, Nell1, Tthy1 and Prss12. Importantly, the testosterone (T) biosynthetic enzymes present in fetal Sertoli cells, Hsd17b1 and Hsd17b3, were identified as activin-responsive. Activin-deficient testes contained elevated androstenedione (A4), displayed an Inhba gene dose-dependent A4/T ratio, and contained 11-keto androgens.

ORGANISM(S): Mus musculus

PROVIDER: GSE143847 | GEO | 2020/04/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of mouse Inhba flox/- Inhbb-/- Amhr2cre/+ activin-deficient granulosa cells vs. wild type

Project description:Compares activin deficient granulosa cells (Inhba flox/-; Inhbb-/-; Amhr2cre/+) to wild type granulosa cells Experiment Overall Design: Three replicate wild type granulosa cell samples compared to 2 activin-deficient granulosa cell samples. Each replicate is derived from 2-3 females.

2008-06-15 | E-GEOD-7150 | biostudies-arrayexpress

Whole transcriptome RNAseq analysis of germ cells in Inhba and Inha fetal testis

Project description:We performed bulk RNA-Seq on male fetal germ cells isolated from E13.5 and E15.5 testes from activin A-deficient mice (Inhba KO), and from E13.5 testes from mice with greater activin A bioactivity (Inha KO). The aim of this expereiment was to determine how chronic altered activin A bioactivity altered the germline transcriptome.

2022-05-26 | GSE201520 | GEO

Exploration the role of INHBA in Hu sheep granulosa cells using RNA-Seq

Project description:Activin/inhibin is an important factor for the fecundity of Hu sheep, and it is involved in follicular development in ovaries. Inhibin subunit beta A (INHBA) participates in the synthesis of activin A and inhibin A. Here we investigated the effect and molecular mechanism of INHBA on cell cycle, apoptosis, activin A/inhibin A secretion in Hu sheep granulosa cells (GCs) based on RNA-Sequencing. A total of 1,687 differentially expressed genes (DEGs) were identified (Fold change ≥ 2 and FDR ≤ 0.01), of which 602 genes were upregulated and 1,087 genes were downregulated in the INHBA interference groups compared with the control groups. Gene Ontology (GO) enrichment indicated that these DEGs were mainly involved in the regulation of cell cycle, protein serine/threonine kinase activity and actin cytoskeleton reorganization. Moreover, pathway analysis showed that DEGs were significantly enriched in 40 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including P53 pathway, progesterone-mediated oocyte maturation, PI3K-AKT signaling pathway. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) confirmed the differential expression of 7 selected DEGs. In addition, INHBA knockdown promoted GCs to G1 phase and decreased the S phase cell numbers, initiated GC apoptosis through P53 signal pathway, and decreased the concentration of activin A and inhibin A in medium. Above all, our findings may contribute to a better understanding on the law of follicular development and thus improve the reproductive performance of female animals.

2025-11-12 | GSE218452 | GEO

INHBA activated PI3K/AKT pathway in gastric cancer cell line MKN45

Project description:The gastric cancer cell' migration ability, invasion ability and the tumor growth could be promoted by INHBA. To investigate the mechanism underlying the regulation of INHBA in promoting gastric cancer progression, high-throughput sequencing was carried in gastric cancer cell line MKN45.The results showed that PI3K/AKT pathway was activated by INHBA. Which indicated that INHBA regulated gastric cancer progression by activating PI3K/AKT pathway.

2026-03-25 | GSE277561 | GEO

Effects of Androstenedione, Ethinyl Estradiol, and Prednisone on Gene Expression in the Male Mouse Liver

Project description:The goal of this study was to provide insights into the adverse outcome pathways that underlie the liver tumor effects of androstenedione in the mouse liver by comparing gene expression patterns to two liver noncarcinogens, ethinyl estradiol and prednisone. B6C3F1 male mice were exposed for 7 days to control diet or diet containing ethinyl estradiol (EE2) at 1, 2.5, 5, or 10 ppm or prednisone (PRED) at 1.25, 2.5, 5, 25 ppm. Androstenedione (A4) was administered in 0.5% carboxymethyl cellulose by gavage at 2, 10, 20, and 50 mg/kg/day. Separate groups of mice were exposed to 50 mg/kg/day A4, 10 ppm EE2 or 25 ppm PRED for 28 days.

2016-07-28 | E-GEOD-84590 | biostudies-arrayexpress

RNA Expression Data from Bovine Ovarian Granulosa Cells from High or Low Androgen-Content Follicles

Project description:Excessive androgen production is a hallmark of human and bovine ovarian disorders, and our lab has identified a naturally-occurring bovine model with excess follicular fluid androstenedione (A4) without a proportional estrogen increase. Granulosa cell populations from both the control, Low A4 follicles and the abnormal, High A4 follicles were isolated. Total RNA was extracted from those granulosa cells and submitted to the University of Nebraska Medical Center Microarray Core Facility for transcriptome quantification using Affymetrix Bovine GeneChip Gene 1.0 ST Arrays. The orignal Affymetrix .CEL files and the normalized linear expression data are included in this submission.

2019-04-30 | GSE97017 | GEO

Palovarotene action against heterotopic ossification includes the inhibition of local participating activin-A expressing cell populations

Project description:Heterotopic ossification (HO) consists of extraskeletal bone formation. One form of HO is acquired and instigated by traumas or surgery, and another form is genetic and characterizes Fibrodysplasia Ossificans Progressiva (FOP). We and others showed that activin A promotes both acquired and genetic HO and found that the retinoid agonist Palovarotene inhibits both HO forms in mice. We asked whether Palovarotene's action against HO may include an interference with endogenous activin A expression and/or function. Using a mouse model of acquired HO, we found that activin A and its encoding RNA (Inhba) were prominent in chondrogenic cells within developing HO masses in untreated mice. Single cell RNAseq (scRNAseq) assays verified that Inhba expression characterized chondroprogenitors and chondrocytes in untreated HO, in addition to its expected expression in inflammatory cells and macrophages. Palovarotene administration caused a sharp inhibition of both HO and amounts of activin A and Inhba transcripts. Bioinformatic analyses of scRNAseq datasets indicated that the drug had reduced interactions and crosstalk amongst cell populations. Our data reveal that Palovarotene markedly reduces the number of local Inhba-expressing HO-forming populations.

2023-07-01 | GSE233843 | GEO

Palovarotene action against heterotopic ossification includes the inhibition of local participating activin A-expressing cell populations

Project description:Heterotopic ossification (HO) consists of endochondral bone forming at extraskeletal anatomical sites. One form of HO is acquired and instigated by traumas or invasive surgery, and another form is genetic and severe and characterizes patients with Fibrodysplasia Ossificans Progressiva (FOP). Previously, we showed that the synthetic retinoid agonist Palovarotene inhibits both acquired and genetic HO, and we and others subsequently showed that systemic administration of a neutralizing activin A antibody reduces both HO forms as well. Here, we asked whether Palovarotene’s action includes an interference with endogenous activin A expression and/or participation in HO. Using a standard mouse model of acquired HO, we found that activin A protein and encoding RNA (Inhba) were prominent in chondrogenic cells within developing HO masses in control untreated mice. Single cell RNAseq (scRNAseq) assays verified that Inhba expression characterized chondroprogenitors and chondrocytes in control HO, in addition to its expected expression in inflammatory cells and macrophages particularly. Palovarotene administration (4 mg/kg/day/gavage) caused a sharp inhibition of both HO and amounts of activin A and Inhba transcripts. Bioinformatic analyses of scRNAseq datasets indicated that the drug had reduced interactions and crosstalk amongst local cell populations. To determine if Palovarotene inhibited Inhba expression directly, we tested it in primary cultures of growth plate chondrocytes. Drug treatment inhibited cartilage phenotypic expression but not Inhba expression. Our data reveal that Palovarotene intersects with activin A during its anti-HO action and markedly reduces the number of local participating Inhba-expressing cell populations. The data broaden the spectrum of HO culprits against which Palovarotene acts, accounting for its therapeutic effectiveness.

2024-06-01 | GSE227253 | GEO

Activin-deficient granulosa cells

Project description:Compares activin deficient granulosa cells (Inhba flox/-; Inhbb-/-; Amhr2cre/+) to wild type granulosa cells Keywords: Genetic Modificaton

2007-12-28 | GSE7150 | GEO

Gene expression profile at single cell level of syngeneic ovarian tumors from mice treated with inhibitory Activin-A antibody or control IgG

Project description:Effective targeting of cancer-associated fibroblasts (CAFs) is hindered by the lack of specific biomarkers and a poor understanding of the mechanisms by which different populations of CAFs contribute to cancer progression. While the role of TGFβ in CAFs is well-studied, less attention has been focused on a structurally and functionally similar protein, Activin A (encoded by INHBA), which is typically associated with poor survival and advanced stage in ovarian cancer. Here, we identified INHBA(+) CAFs as key players in tumor promotion and immune evasion. In syngeneic ovarian cancer mouse models, intraperitoneal injection of the Activin A neutralizing antibody attenuated tumor progression and infiltration with the host INHBA(+) CAFs and M2 macrophages. Collectively, our study identified an INHBA(+) subset of pro-tumoral CAFs as a potential therapeutic target in ovarian cancer.

2023-05-01 | GSE229529 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data