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Genome-wide maps of chromatin state in WT c-Myc and K427R mutant lymphoma cells

ABSTRACT: We report the application of single-molecule-based sequencing technology for high-throughput profiling of c-Myc with core promoters of its target genes in mammalian cells. We performed ChIP sequencing with antibody to total c-Myc with core promoters of its target genes in WT c-Myc and K427R mutant group. Inspection of multiple individual traces and global analyses showed that the K427R mutant globally reduced association of c-Myc with core promoters of its target genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE143864 | GEO | 2020/10/10

REPOSITORIES: GEO

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Genome-wide maps of chromatin state in WT c-Myc and K427R mutant lymphoma cells

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of c-Myc with core promoters of its target genes in mammalian cells. We performed ChIP sequencing with antibody to total c-Myc with core promoters of its target genes in WT c-Myc and K427R mutant group. Inspection of multiple individual traces and global analyses showed that the K427R mutant globally reduced association of c-Myc with core promoters of its target genes.

2020-10-02 | GSE126744 | GEO

Genome-wide maps of chromatin state in CTRLcas9 and SCARB2cas9 HepG2 cells.

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of c-Myc with core promoters of its target genes in mammalian cells. We performed ChIP sequencing with antibody to total c-Myc with core promoters of its target genes in CTRLcas9 and SCARB2cas9 group. Inspection of multiple individual traces and global analyses showed that SCARB2cas9 globally reduced association of c-Myc with core promoters of its target genes.

2023-08-11 | GSE185838 | GEO

CUT&Tag analysis genome-wide maps of chromatin state in CTRLcas9 and SCARB2cas9 HepG2 cells

Project description:We report the application of CUT&Tag sequencing technology for high-throughput profiling of c-Myc with core promoters of its target genes in mammalian cells. We performed CUT&Tag sequencing with antibody to total c-Myc with core promoters of its target genes in CTRLcas9 and SCARB2cas9 group. Inspection of multiple individual traces and global analyses showed that SCARB2cas9 globally reduced association of c-Myc with core promoters of its target genes.

2023-08-11 | GSE207672 | GEO

Genome-wide maps of chromatin state in TRIB3Cas9 and control lymphoma cells

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of RNAPII with promoters in mammalian cells. We performed ChIP sequencing with antibody to total RNAPII in TRIB3Cas9 and CtrlCas9group. Inspection of multiple individual traces and global analyses showed that TRIB3 deletion globally reduced association of RNAPII with promoters.

2020-10-10 | GSE143866 | GEO

Genome-wide maps of chromatin state in TRIB3-shRNA and control lymphoma cells

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of RNAPII with promoters in mammalian cells. We performed ChIP sequencing with antibody to total RNAPII in TRIB3-cas9 and control group. Inspection of multiple individual traces and global analyses showed that TRIB3 deletion globally reduced association of RNAPII with promoters.

2020-10-02 | GSE126743 | GEO

Genome-wide maps of chromatin state in Ad-UBE3B and control lymphoma cells

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of RNAPII with promoters in mammalian cells. We performed ChIP sequencing with antibody to total RNAPII in UBE3B overexpression and control group. Inspection of multiple individual traces and global analyses showed that UBE3B overexpression globally reduced association of RNAPII with promoters.

2020-10-10 | GSE143863 | GEO

Genome-wide maps of chromatin state in Pep2-CM4-treated and control lymphoma cells

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of RNAPII with promoters in mammalian cells. We performed ChIP sequencing with antibody to total RNAPII in Pep2-CM4-treated and control group. Inspection of multiple individual traces and global analyses showed that Pep2-CM4-treated cells globally reduced association of RNAPII with promoters.

2020-10-10 | GSE143865 | GEO

Genome-wide maps of chromatin state in Ad-ube3b and control lymphoma cells

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of p-RNAPII(ser2) with promoters in mammalian cells. We performed ChIP sequencing with antibody to total p-RNAPII(ser2) in UBE3B overexpression and control group. Inspection of multiple individual traces and global analyses showed that UBE3B overexpression globally reduced association of p-RNAPII(ser2) with promoters.

2020-10-02 | GSE126746 | GEO

Genome-wide maps of chromatin state in ad-ube3b or Pep2-CM4-treated and control lymphoma cells

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of RNAPII with promoters in mammalian cells. We performed ChIP sequencing with antibody to total RNAPII in UBE3B overexpression or Pep2-CM4-treated and control group. Inspection of multiple individual traces and global analyses showed that UBE3B overexpression globally reduced association of RNAPII with promoters.

2020-10-02 | GSE126745 | GEO

Analysis of MIZ1, MYC-ER and MYC-ERVD binding sites in MCF10A cells

Project description:Oncogenic levels of Myc expression sensitize cells to multiple apoptotic stimuli and this protects long-lived organisms from cancer development. How cells discriminate physiological from supra-physiological levels of Myc is largely unknown. Here we show that induction of apoptosis by Myc in breast epithelial cells requires association of Myc with Miz1. Gene expression and ChIP-sequencing experiments show that oncogenic levels of Myc, but not of MycV394D, a point mutant that does not bind Miz1, recruit Miz1 to core promoters and enable binding of Myc/Miz1 complexes to low-affinity target sites, correlating with repression of a specific set of target genes. Repressed genes encode proteins involved in cell adhesion, migration and wound healing; their promoters are enriched for binding sites of the serum response (SRF) factor. Restoring SRF activity attenuates Myc-induced apoptosis in response to glutamine starvation, exposure to Trail and to DNA damage. We propose that supra-physiological levels of Myc engage Miz1 in repressive DNA binding complexes and suppress transcriptional progress.

2015-07-10 | GSE59146 | GEO

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