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Transcriptional regulation of cell division and cytokinin response in Arabidopsis shoot apical meristem [ChIP-seq]

ABSTRACT: In plants, the phytohormone cytokinin plays a major role in promoting cell division. However, the molecular mechanisms underlying cytokinin stimulated cell proliferation remain poorly understood. Here we show that, in the meristems of Arabidopsis thaliana, two transcriptional factors, MYB3R1 and MYB3R4, are master regulators of mitotic cell cycle gene expression and cytokinin response.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE144047 | GEO | 2021/03/26

REPOSITORIES: GEO

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Transcriptional regulation of cell division and cytokinin response in Arabidopsis shoot apical meristem [RNA-seq]

Project description:In plants, the phytohormone cytokinin plays a major role in promoting cell division. However, the molecular mechanisms underlying cytokinin stimulated cell proliferation remain poorly understood. Here we show that, in the meristems of Arabidopsis thaliana, two transcriptional factors, MYB3R1 and MYB3R4, are master regulators of mitotic cell cycle gene expression and cytokinin response.

2021-03-26 | GSE144048 | GEO

Transcriptional regulation of cell division and cytokinin response in Arabidopsis shoot apical meristem

Project description:Transcriptional regulation of cell division and cytokinin response in Arabidopsis shoot apical meristem

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Transcriptional regulation of cell division and cytokinin response in Arabidopsis shoot apical meristem

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2021-03-26 | GSE144049 | GEO

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Transcriptional regulation of cell division and cytokinin response in Arabidopsis shoot apical meristem [ChIP-seq]

Project description:Transcriptional regulation of cell division and cytokinin response in Arabidopsis shoot apical meristem [ChIP-seq]

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Gene Expression between two cell layers in maize shoot apical meristem

2013-01-28 | GSE16868 | GEO

Proteome of rice shoot apical meristem

Project description:DNA methylation is an epigenetic modification that specifies the basic state of pluripotent stem cells and regulates the developmental transition from stem cells to various cell types. In flowering plants, the shoot apical meristem (SAM) contains a pluripotent stem cell population which generates the aerial part of plants including the germ cells. Under appropriate conditions, the SAM undergoes a developmental transition from a leaf-forming vegetative SAM to an inflorescence- and flower-forming reproductive SAM. While SAM characteristics are largely altered in this transition, the complete picture of DNA methylation remains elusive. Here, by analyzing whole-genome DNA methylation of isolated rice SAMs in the vegetative and reproductive stages, we found that methylation at CHH sites is kept high, particularly at transposable elements (TEs), in the vegetative SAM relative to the differentiated leaf, and increases in the reproductive SAM via the RNA-dependent DNA methylation pathway. We also found that half of the TEs that were highly methylated in gametes had already undergone CHH hypermethylation in the SAM. Our results indicate that changes in DNA methylation begin in the SAM long before germ cell differentiation to protect the genome from harmful TEs.

2020-08-14 | PXD020608 | Pride

Gene Expression between two cell layers in maize shoot apical meristem

Project description:All above ground organs of higher plants are ultimately derived from shoot apical meristems (SAMs). The SAM exhibits distinctive structural organization, and monocot SAMs such as maize are comprised of two cell layers, a single cell layered tunica (L1) and a corpus (L2). Although recent research has revealed roles of these cell layers in the SAM, intra- and inter-cell-layer signaling networks involved in organ development remain largely unknown except for a few differentially expressed genes. Here, we used Illumnia technology to conduct RNA-seq of L1 and L2 cell layers in maize B73 maize shoot apical meristem. Single sequencing library was constructed for L1 and L2 cell layer. Each library was sequenced using 2 lanes on a Solexa flow cell. Processed data file 'ZmB73_4a.53_filtered_genes.fasta' and its README file are linked below as supplementary files. The fasta file contains the gene model ID and corresponding sequence generated from maize genome project. This fasta file was used for the following samples: GSM418173, GSM418174, GSM420173, GSM420174, GSM422828, GSM422829.

2013-01-28 | E-GEOD-16868 | biostudies-arrayexpress

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