Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differential transcriptional expression by Babesia bovis during parasite development within bovine erythrocytes and tick hemolymph

ABSTRACT: Babesia parasites transition between a mammalian host, where they cause babesiosis, and the tick vector that transmits them. This transition provides an environmental signal resulting in altered gene expression allowing the completion of the parasite’s life cycle. A comparison of the different life stages that occur within mammalian and tick hosts can provide insight into the adaptation of Babesia to these different environments. In this study, we used RNA-Seq to compare gene expression between Babesia bovis blood stages and tick derived kinetes.

ORGANISM(S): Babesia bovis

PROVIDER: GSE144066 | GEO | 2020/10/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Comparison of high throughput RNA sequences between Babesia bigemina and Babesia bovis revealed consistent differential gene expression that is required for Babesia life cycle in the vertebrate and invertebrate hosts.

Project description:To understand Babesia gene regulation during tick and mammalian host infection, we performed high throughput RNA-sequencing using samples collected from calves and Rhipicephalus microplus ticks infected with Babesia bigemina. We evaluated gene expression differences between B. bigemina kinetes and blood-stage parasites

2022-12-20 | GSE214115 | GEO

Differential transcriptional expression by Babesia bovis during parasite development within bovine erythrocytes and tick hemolymph

Project description:Differential transcriptional expression by Babesia bovis during parasite development within bovine erythrocytes and tick hemolymph

| PRJNA602683 | ENA

Morphological and molecular descriptors of the developmental cycle of Babesia divergens parasites in human erythrocytes

Project description:Human babesiosis, especially caused by the cattle derived Babesia divergens parasite, is on the increase, resulting in renewed attentiveness to this potentially life threatening emerging zoonotic disease. The molecular mechanisms underlying the pathophysiology and intra-erythrocytic development of these parasites are poorly understood. This impedes concerted efforts aimed at the discovery of novel anti-babesiacidal agents. By applying sensitive cell biological and molecular functional genomics tools, we describe the intra-erythrocytic development cycle of B. divergens parasites from immature, mono-nucleated ring forms to bi-nucleated paired piriforms and ultimately multi-nucleated tetrads that characterizes zoonotic Babesia spp. This is further correlated for the first time to nuclear content increases during intra-erythrocytic development progression, providing insight into the part of the life cycle that occurs during human infection. High-content temporal evaluation elucidated the contribution of the different stages to life cycle progression. Moreover, molecular descriptors indicate that B. divergens parasites employ physiological adaptation to in vitro cultivation. Additionally, differential expression is observed as the parasite equilibrates its developmental stages during its life cycle. Together, this information provides the first temporal evaluation of the functional transcriptome of B. divergens parasites; information that could be useful in identifying biological processes essential to parasite survival for future anti-babesiacidal discoveries. Two-condition experiment, Untreated vs.Treated B. divergens parasites, cultured in human erythrocytes. Treatment with a piperidinyl-benzimidizalone analogue. Biological replicates: 3 untreated (control) replicates, 3 treated replicates. The 6-sample dataset represents untreated(control) vs pooled_reference samples at various timepoints.

2015-04-10 | E-GEOD-67677 | biostudies-arrayexpress

Morphological and molecular descriptors of the developmental cycle of Babesia divergens parasites in human erythrocytes

2015-04-10 | GSE67677 | GEO

Rhipicephalus microplushemocyte innate cellular and humoral immune responses to babesialinfection

Project description:To understand the effect of Babesia infection on Rhipicephalus microplus hemocyte gene expression, we performed high throughput RNA-sequencing using samples collected from Rhipicephalus microplus uninfected tick hemolymph and infected with either Babesia bigemina or Babesia bovis. We evaluated gene expression differences that may be attributed to tick immune defense to babesial infection.

2023-12-31 | GSE243493 | GEO

Tick Adiponectin Receptor-Mediated Phospholipid Metabolism Contributes to Borrelia burgdorferi Colonization

Project description:Adiponectin-mediated pathways contribute to mammalian homeostasis; however, little is known about adiponectin and adiponectin receptor signaling in arthropods. In this study, we demonstrate that Ixodes scapularis ticks have an adiponectin receptor-like protein (ISARL) but lack adiponectin, suggesting activation by alternative pathways. ISARL expression is significantly upregulated in the tick gut after Borrelia burgdorferi infection, suggesting that ISARL signaling may be co-opted by the Lyme disease agent. Consistent with this, RNA interference (RNAi)-mediated silencing of ISARL significantly reduced the B. burgdorferi burden in the tick. RNA-seq-based transcriptomics and RNAi assays demonstrate that ISARL-mediated phospholipid metabolism by phosphatidylserine synthase I is associated with B. burgdorferi survival. Furthermore, the tick complement C1q-like protein 3 interacts with ISARL, and B. burgdorferi facilitates this process. This study identifies a new tick metabolic pathway that is connected to the life cycle of the Lyme disease spirochete.

2021-12-08 | GSE169293 | GEO

Comparative transcriptome analysis of geographically distinct virulent and attenuated Babesia bovis

Project description:Geographical distinct virulent Babesia bovis strains have similar gene expression changes as they go through attenuation. Pair end RNA-sequencing reads on three biological replicate sample pairs of virulent parent and attenuated derivative Babesia bovis strain isolated in Argentina.

2013-10-23 | E-GEOD-51560 | biostudies-arrayexpress

Tick

Project description:Tick

| PRJEB21763 | ENA

Comparative transcriptome analysis of geographically distinct virulent and attenuated Babesia bovis

Project description:Geographical distinct virulent Babesia bovis strains have similar gene expression changes as they go through attenuation.

2013-10-23 | GSE51560 | GEO

Babesia

Project description:Babesia MLST scheme

| PRJNA170021 | ENA