RNA-seq analysis of transcriptomes in control and LAMP2A-overexpressed D3 ES cells

ABSTRACT: The role of autophagy and metabolism in regulating the pluripotency of stem cells is not well understood. LAMP2A is a key gene in CMA. Here we compared transcriptomic changes in LAMP2A-overexpressed D3 versus control cells, as well as changes in LAMP2A-overexpressed cells with versus without DM-αKG treatment (4mM, 72 hrs). We found that overexpression of LAMP2A leads to decreased expression of genes related to stem cell differentiation, and this can be largely restored by DM-αKG supplement. This study provides a promising link for autophagy, metabolism, and pluripotency of stem cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE144093 | GEO | 2020/11/08

REPOSITORIES: GEO