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Cellular interaction with S. aureus bacteria or with bacterial supernatant

ABSTRACT: Each condition (cellular interaction with bacteria or with bacterial supernatant) was hybridized on 6 slides (3 slides, corresponding to different interactions and RNA extractions, were swapped). Keywords: parallel sample

ORGANISM(S): Homo sapiens

PROVIDER: GSE1444 | GEO | 2004/06/03

SECONDARY ACCESSION(S): PRJNA89991

REPOSITORIES: GEO

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Cellular interaction with S. aureus bacteria or with bacterial supernatant

Project description:Each condition (cellular interaction with bacteria or with bacterial supernatant) was hybridized on 6 slides (3 slides, corresponding to different interactions and RNA extractions, were swapped).

2010-06-09 | E-GEOD-1444 | biostudies-arrayexpress

interaction with bacteria or bacterial supernatant

Project description:Epithelial cells were in contact either with live bacteria (S. aureus) or with bacterial supernatant during 3 hours. Three dependant experiment for each condition were performed. RNA from epithelial cells were extracted. Each condition was hybridized on 6 slides (3 slides with their corresponding swap) Keywords: parallel sample

2004-10-23 | GSE1853 | GEO

interaction with bacteria or bacterial supernatant

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Epithelial cells in contact with bacterial supernatant

Project description:Epithelial cells were in contact with bacteria supernatant during different times of incubation. time course supernatant 10 % MM39 Keywords: time-course

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Epithelial cells in contact with bacterial supernatant

Project description:Epithelial cells were in contact with bacteria supernatant during different times of incubation. time course supernatant 10 % MM39

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Pseudomonas aeruginosa gene regulation by Staphylococcus aureus supernatant or added secreted products

Project description:Pseudomonas aeruginosa and Staphylococcus aureus are often co-isolated in persistent infections. The goal of this study was to determine how secreted products that were identified in S. aureus supernatant affect gene expression in P. aeruginosa. Therefore, media control, the indicated products in media, or S. aureus supernatant was added to P. aeruginosa cultures at 25% total volume and gene expression was measured at 20 min and 2 h using RNA-seq. The individual products induced distinct pathways in P. aeruginosa. The products in combination recapitulated much of the differential gene expression seen in P. aeruginosa in response to S. aureus supernatant.

2022-05-18 | GSE186138 | GEO

Gene expression analysis of Staphyloccoccus aureus USA300 vs USA300 sae knockout

Project description:The study aims to compare gene expression patterns of Staphyloccoccus aureus USA300 vs USA300 sae knockout. Samples were hybridized on aminosilane coated slides with 70-mer oligos.

2011-04-08 | GSE26243 | GEO

Synechocystis PCC6803 time course responses to cadmium (CdSO4, 50 microM, 300 min.

Project description:The transcriptome approach was used to characterize the of the global change in Synechocystis gene expression in response to continuous exposure to cadmium (50 microM). Total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays. Keywords: stress responses

2005-12-07 | GSE3718 | GEO

Time course response of Synechocystis PCC 6803 to dehydration/desiccation

Project description:The responses of the transcriptome were monitored in Synechocysis PCC 6803 during a linear rate of evaporation of the culture to dryness (desiccation). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays. Keywords: Dehydration, stress response, time course, transcription, cyanobacteria

2006-09-04 | GSE4604 | GEO

Synechocystis PCC6803 time course responses to zinc (ZnSO4, 776 microM.

Project description:The transcriptome approach was used to characterize the of the global change (2 time points) in Synechocystis gene expression in response to continuous exposure to zinc excess (776 microM), compared to standard medium (776 nM). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays. Keywords: stress responses

2005-12-07 | GSE3716 | GEO

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