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Changes in gene expression in MKK2 and MKKKC5 mutants: wt vs mutant comparison

ABSTRACT: The mkkkC5 mutant, a knockout line of MAPKKKC5, shows changes in growth and development, as well as an altered Alternaria sensitivity. It was shown by Brader and Djamei et al.(2007) that Alternaria brassicicola sensitivity is also influenced by MKK2-activity. Aim of this experiment is to access the transcriptional changes in the mkkkc5plants as well as in plants overexpressing a constitutively active MKK2-version (MKK2EE). The second part of the experiment addresses transcriptional changes in these plants after Alternaria brassicicola infection. - au07-09_alternaria - mkk2ee and mkkkc5 mutants Keywords: wt vs mutant comparison

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE14444 | GEO | 2009/02/15

SECONDARY ACCESSION(S): PRJNA114499

REPOSITORIES: GEO

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Changes in gene expression in MKK2 and MKKKC5 mutants after Alternaria infection

2009-02-15 | GSE14443 | GEO

Changes in gene expression in MKK2 and MKKKC5 mutants after Alternaria infection

Project description:The mkkkC5 mutant, a knockout line of MAPKKKC5, shows changes in growth and development, as well as an altered Alternaria sensitivity. It was shown by Brader and Djamei et al.(2007) that Alternaria brassicicola sensitivity is also influenced by MKK2-activity. Aim of this experiment is to access the transcriptional changes in the mkkkc5plants as well as in plants overexpressing a constitutively active MKK2-version (MKK2EE). The second part of the experiment addresses transcriptional changes in these plants after Alternaria brassicicola infection. au07-09_alternaria Keywords: treatment variations Alternaria brassicicola infection of 25 day old mkkkc5- , pGreen::MKK2EE-myc and WT Col-0 plants. Sampling after 0, 1 and 2 days. Comparison between mutants and WT at corresponding timepoints. Additionally comparison between WT-samples at different timepoints. 16 dye-swap - CATMA arrays

2009-02-15 | E-GEOD-14443 | biostudies-arrayexpress

Changes in gene expression in MKK2 and MKKKC5 mutants: wt vs mutant comparison

Project description:The mkkkC5 mutant, a knockout line of MAPKKKC5, shows changes in growth and development, as well as an altered Alternaria sensitivity. It was shown by Brader and Djamei et al.(2007) that Alternaria brassicicola sensitivity is also influenced by MKK2-activity. Aim of this experiment is to access the transcriptional changes in the mkkkc5plants as well as in plants overexpressing a constitutively active MKK2-version (MKK2EE). The second part of the experiment addresses transcriptional changes in these plants after Alternaria brassicicola infection. - au07-09_alternaria - mkk2ee and mkkkc5 mutants Keywords: wt vs mutant comparison Comparison between the over-expressed mutant of the MKK2 gene and the KO mutant for the MKKKC5 gene versus the wild type. 4 dye-swap - CATMA arrays

2009-02-15 | E-GEOD-14444 | biostudies-arrayexpress

Changes in gene expression in MKK2 and MKKKC5 mutants

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Project description:au08-02_mkk1-mkk2 - mkk1-mkk2 - Functional redundancy of the two MAPKKs MKK1 and MKK2. - mkk1 and mkk2 k.o. plants were crossed. Seedlings homozygous for mkk1 and/or mkk2, in parallel with control wild type seedlings, were grown on sterile medium. RNA was extracted from non-treated seedlings. aRNA was synthesised using the Ambion aRNA kit. Transcriptome data will be compared with those from a (already available) microarray of a mutant lacking the MAPKKkinase that is upstream of MKK1 and MKK2 (MEKK1).

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Transcriptome of mkk1-mkk2 mutants

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Invasion by diverse fungal pathogens in Arabidopsis thaliana commonly activates both tryptophan-dependent and independent defense pathways exhibiting distinct sensitivity to the bak1-5 mutation

Project description:Transcriptome analysis to identify genes up- and down-regulated genes in pen2 bak1-5 plants compared with pen2 plants in the case of Alternaria brassicicola (Ab) inoculation .

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Transcription profiling of Arabidopsis mkk1, mkk2 and mkk1/2 knockout treated with the saliicylic acid analog BTH

Project description:Gene Expression profiling of the Arabidopsis thaliana MAP Kinase Kinases 1 (mkk1), MAP Kinase Kinases 2 (mkk2) knockout mutants and the double mutant mkk1/mkk2 before and 24 hours after treatment with the salicylic acid analog BTH, was measured by hybridisation to an Affymetrix ATH1 GeneChip. Experiment Overall Design: Whole Arabidopsis thaliana mkk1, mkk2 and mkk1/mkk2 knockout mutants and wild type plants (Col-0) were sampled in triplicates before and 24 hours after BTH treatment.

2008-06-11 | E-GEOD-10646 | biostudies-arrayexpress

mkk2_mkk2_and_wrky19_cold_time_course-Transcriptomic analysis of mkk2 upon Cold stress

Project description:adt06-03-mkk2 - mkk2_mkk2_and_wrky19_cold_time_course - early response targets of MKK2 upon cold stress treatment and its role in adaptation. - seedlings were put to 4 ° comparing wild type ColO versus mkk2 knockouts, MKK2 overexpressors and wrky19 knockouts. Keywords: time course,wt vs mutant comparison

2008-07-01 | GSE10763 | GEO

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