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Transcriptomic profiling of wild type, sigG1 and rsfG mutants of Streptomyces tsukubaensis in liquid culture

ABSTRACT: The aim of this work was to compare gene expression of wild type, sigG1 and rsfG mutants, to determine the SigG1-dependent genes in Streptomyces

ORGANISM(S): Streptomyces tsukubensis NRRL18488

PROVIDER: GSE144815 | GEO | 2020/12/21

REPOSITORIES: GEO

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Transcriptomic profiling of wild type, sigG1 and rsfG mutants of Streptomyces tsukubaensis in liquid culture

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Project description:In this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcriptomic profiling of S. tsukubaensis growing under tacrolimus producing and non-producing conditions. The analysis revealed important and different metabolic changes after the additions and a lack of transcriptional activation of the fkb cluster. In addition, we detected important differences in the transcriptional response to glucose between S. tsukubaensis and the model species Streptomyces coelicolor. A number of genes encoding key players of morphological and biochemical differentiation were strongly and permanently downregulated by the carbon sources. Finally, we identified several genes showing transcriptional profiles highly correlated to that of the tacrolimus biosynthetic pathway regulator FkbN that might be potential candidates for the improvement of tacrolimus production

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