Understanding the roles of dksA and greA in inorganic polyphosphate accumulation in Escherichia coli

ABSTRACT: Inorganic polyphosphate (polyP) is synthesized by bacteria in response to various stresses, but the mechanism of its regulation is unknown. Mutants of Escherichia coli lacking the RNA polymerase-binding transcription factor dksA are defective in polyP synthesis after a nutrient limitation stress, and this defect is reversed in a dksA greA mutant. In this work, we used RNA sequencing to compare transcription in wild-type, dksA, and dksA greA strains of E. coli before and after nutrient limitation, to identify genes whose expression pattern correlates with ability to synthesize polyP.

ORGANISM(S): Escherichia coli

PROVIDER: GSE144816 | GEO | 2020/03/11

REPOSITORIES: GEO