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Transcriptome sequencing of epididymal adipose tissue harvested from 2-month-old Tfrcfl/fl and TfrcAKO mice on high fat diet for 10 more weeks

ABSTRACT: Purpose: The goals of this study are to compare the transcription difference of epididymal fat between Tfrcfl/fl and TfrcAKO mice after high fat diet treatment Methods: Transcriptome of epididymal adipose tissue was generated by deep sequencing, using Illumina Novaseq 6000 sequencing system.

ORGANISM(S): Mus musculus

PROVIDER: GSE144938 | GEO | 2021/06/25

REPOSITORIES: GEO

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Transcriptome sequencing of epididymal adipose tissue harvested from 2-month-old Tfrcfl/fl and TfrcAKO mice on high fat diet for 10 more weeks

Project description:Transcriptome sequencing of epididymal adipose tissue harvested from 2-month-old Tfrcfl/fl and TfrcAKO mice on high fat diet for 10 more weeks

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Identification of genes characteristic of primary inguinal or epididymal preadipocyte fibroblasts

Project description:Committed preadipocyte fibroblasts were genetically labelled in transgenic mice by expressing GFP under the control of the locus for Zfp423, a gene controlling preadipocyte determination. These mice are herein referred to as Zfp423-GFP mice. The overall goal was to identify genes differentially expressed between adipogenic GFP+ firboblasts and non-adipogenic GFP- fibroblasts from either inguinal or epididymal fat stromal vascular cultures obtained from Zfp423-GFP mice. Adipose stromal vascular cultures were obtained from inguinal or epididymal fat of 6-8 week-old male Zfp423-GFP mice. GFP+ and GFP- fibroblasts were sorted from fat stromal vascular cultures and then returned to culture. Subconfluent cultures of GFP+ and GFP- cells were compared in triplicate for Affymetrix analysis.

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Transcription profiling of rat Perirenal and epididymal preadipocytes isolated from old and young individuals

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Expression data from mice epididymal adipose tissue

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Gene expression data from epididymal fat of A/J mouse and A/J-12SM consomic mouse

Project description:SMXA-5 mouse shows a high-fat induced fatty liver. The QTL for fatty liver was mapped on mouse chromosome 12, designated as Fl1sa. The liver weight and liver triglyceride content of A/J-12SM consomic mouse was lower than that of A/J mouse. In contrast, the epididymal fat weight tended to be higher in A/J-12SM consomic mouse than that in A/J. We compared the epididymal fat's gene expressions between A/J mouse and A/J-12SM consomic mouse.

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Expression data from epididymal fat tissues of diet induced obese rats

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Gene expression data from epididymal fat of systemic Iah1-knockout in A/J-12SM mouse

Project description:The QTL for fatty liver was mapped on mouse chromosome 12, designated as Fl1sa. Iah1 gene is a candidate gene for Fl1sa . In mammal, Iah1 function has been largely unknown. We constructed Iah1-knockout mouse in A/J-12SM background. Iah1 gene is abundantly expressed in epididymal fat of mouse. We compared the epididymal fat's gene expressions between Iah1-WT mouse and Iah1-KO mouse.

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mRNA expression data in epididymal fat tissues derived from mir342-/- and mir342wt/wt mice

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