Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Decoding drought tolerance mechanisms in Holm oak (Quercus ilex) throught a combined transcriptomics and proteomics analysis

ABSTRACT: Transcript profiling of leaves from Quercus ilex seedlings subjected to well-watering and drought-stress (irrigation withdrawal) conditions

ORGANISM(S): Quercus ilex

PROVIDER: GSE145009 | GEO | 2020/04/03

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Decoding drought tolerance mechanisms in Holm oak (Quercus ilex) throught a combined transcriptomics and proteomics analysis

Project description:Drought is considered as a threat to the sustainability of Mediterranean forests and, of the agro-silvo-pastoral “dehesa” system. Holm oak (Quercus ilex L.) is one of the most representative species of these ecosystems and this abiotic stress causes a high tree mortality rate, being more accused at the seedling stage. In this study, an integrative analysis of transcriptome and proteome in leaves of Q. ilex seedlings under drought conditions was carried out to identify gene products involved in the metabolic pathways related to drought tolerant mechanisms.

2022-11-11 | PXD017493 | Pride

Decoding drought tolerance mechanisms in Holm oak (Quercus ilex) throught a combined transcriptomics and proteomics analysis

Project description:Decoding drought tolerance mechanisms in Holm oak (Quercus ilex) throught a combined transcriptomics and proteomics analysis

| PRJNA605710 | ENA

Transcription profiling of Arabidopsis dor mutant and wild type plants in response to drought stress.

Project description:ATH1 GeneChip was used for gene expression analysis of wild-type plants and dor mutant under drought treatment (both the wild-type and dor plants were grown under normal watering conditions for 24 days and then stressed by completely depriving of irrigation for 10 days). Two biological repeat experiments were conducted and the raw data was analyzed applying Affymetrix GCOS software. Experiment Overall Design: ATH1 GeneChip was used for gene expression analysis of wild-type plants and dor mutant under drought treatment (both the wild-type and dor plants were grown under normal watering conditions for 24 days and then stressed by completely depriving of irrigation for 10 days).

2008-10-26 | E-GEOD-10643 | biostudies-arrayexpress

Transcription profiling of Arabidopsis dor mutant and wild-type plants in response to drought stress.

Project description:ATH1 GeneChip was used for gene expression analysis of wild-type plants and dor mutant under drought treatment (both the wild-type and dor plants were grown under normal watering conditions for 24 days and then stressed by completely depriving of irrigation for 10 days). Two biological repeat experiments were conducted and the raw data was analyzed applying Affymetrix GCOS software. Keywords: both the wild-type and dor plants were grown under normal watering conditions for 24 days and then stressed for 10 days

2008-09-25 | GSE10643 | GEO

The transcriptome of Oak roots associated with truffles

Project description:Illumina HiSeq technology was used to generate mRNA profiles from mycorrhizal Quercus robur roots. Tuber melanosporum, T. aestivum and T.magnatum mycorrhizal root tips were harvested and used for RNA extraction. Paired-end reads of 100 bp were generated and aligned to Quercus robur CDS using CLC Genomics Workbench 9.

2018-07-01 | GSE97122 | GEO

2-DE proteomics analysis of drought treated seedlings of Quercus ilex

Project description:Applying a gel-based proteomic approach, the dynamic changes in root proteins of drought treated Quercus ilex subsp. Ballota [Desf.] Samp. seedlings were followed. Water stress was applied on 20 day-old holm oak plantlets by water limitation for a period of 10 and 20 days, each followed by 10 days of recovery. Root proteins were extracted using trichloroacetate/acetone/phenol protocol and subjected to two-dimensional electrophoresis. Coomassie colloidal stained gel images were analysed and spot intensity data subjected to multivariate statistical analysis. Selected consistent spots in the three biological replicas, presenting significant changes under stress, were subjected to MALDI-TOF mass spectrometry (peptide mass fingerprinting and MS/MS). For protein identification, combined search was performed with MASCOT search engine over NCBInr Viridiplantae and Uniprot databases. Taxonomy Holm oak (Quercus ilex subsp. Ballota [Desf.] Samp.). Dentro de Q. ilex hay dos subespecies, ilex ilex e ilex Ballota.

2015-09-09 | PXD002484 | Pride

Assessment of shared alleles in drought-associated candidate genes among southern California white oak species (Quercus sect. Quercus).

Project description: Not available

| S-EPMC6167808 | biostudies-literature

Proteomic response to drought and Phytophthora cinnamomi in Quercus ilex.

Project description:Biotic and abiotic stresses are predicted to be the main drivers of forest tree decline and mortality in a climatic change scenario. It occurs, for example, in Quercus ilex, the main component of the Mediterranean forest, becoming a major environmental, economic and social concern. The decline syndrome is the result of factors interrelated in time and space. Breeding, through variability and elite genotype selection, is the only plausible strategy for forest management and conservation in nondomesticated, orphan species as is the case of the genus Quercus. By using a shotgun proteomics approach, the effect and the responses to combined drought stress and pathogen (Phytophthora cinnamomi) have been analyzed in two Holm oak populations with contrasting responses to each individual stress. Proteomics data are correlated with that of plant survival, damage symptoms, leaf chemical composition and physiological phenotypes (water content and photosynthetic activity). Changes in the protein profile depended on the population, stress, and time, with some consistent upaccumulated proteins related to synthesis, primary and secondary metabolism, and cell wall are discussed.

2021-08-18 | PXD025704 | Pride

The effect of drought stress on heterozygosity-fitness correlations in pedunculate oak (Quercus robur).

Project description: Not available

| S-EPMC3997642 | biostudies-literature

Quercus

Project description:Quercus ilex, Quercus pubescens, Quercus robur Assembly

| PRJNA450334 | ENA