Identifying cleaved and noncleaved targets of small interfering RNAs and microRNAs in mammalian cells by SpyCLIP

ABSTRACT: we report a strategy to establish a comprehensive picture of siRNA cleaved and noncleaved off-targets by performing SpyCLIP using wild-type and catalytically inactive AGO2 mutants in parallel. Additionally, we investigated naturally occurring cleavage events mediated by endogenous miRNAs using the same strategy. Our results demonstrated that AGO2 SpyCLIP is a powerful method to identify both cleaved and noncleaved targets of siRNAs, providing valuable information for improving siRNA design rules

ORGANISM(S): Homo sapiens

PROVIDER: GSE145438 | GEO | 2020/12/01

REPOSITORIES: GEO