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Lens epithelial cell transcriptome reprogramming at 24 hours post cataract surgery (PCS) in wild type (WT) and β8 integrin Conditional Knockout (β8ITGcKO) mice.

ABSTRACT: This trancriptome analysis reveals the first detailed analysis on how the remnant lens epithelial cells (LECs) greatly reprogram their transcriptome by 24 hours post cataract surgery (PCS) and the dependence of this phenomenom on endogenous β8 integrin gene expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE145492 | GEO | 2021/12/15

REPOSITORIES: GEO

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Lens epithelial cell transcriptome reprogramming at 24 hours post cataract surgery

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Expression data from lens epithelial cells (LECs) from Shumiya cataract rats (SCR) with or without cataract (Cat+ or Cat-)

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Identification and Characterization of N6-Methyladenosine CircRNAs and Methyltransferases in the Lens Epithelium Cells from Age-related Cataract

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Project description:The purpose of this study was to investigate/characterize age- and sex-dependent changes in transcriptomic profile in a mouse model of cataract surgery that mimics human cataract surgery. This surgery involves the removal of lens fiber cells (LFC) leaving behind the lens capsule and associated lens epithelial cells (LEC). The lens capsule and associated remnant LECs were harvested either following cataract surgery (0 hour) or 24 hours later. Fiber cells were collected at the time of surgery, equivalent to 0 hour, and analyzed separately from epithelial cells. For samples from LEC, each biological replicate is a pool of 5 lens capsules from 5 independent mice. For samples from LFC, each biological replicate is a pool of 2 fiber cell masses from two independent mice. Mice were either 24 months old (Aged), or 3 months old (Young).

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