Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MicroRNA profiling of exosomes derived from human pulmonary artery smooth muscle cells under PDGF stimulation

ABSTRACT: Analysis of exosomal microRNAs secreted by PDGF-stimulated human pulmonary artery smooth muscle cells. Results increase our understanding of exosome-mediated crosstalk between vascular cells under a pathological condition.

ORGANISM(S): Homo sapiens

PROVIDER: GSE145508 | GEO | 2020/04/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

microRNA profiling of exosomes derived from human pulmonary artery smooth muscle cells under PDGF stimulation

Project description:microRNA profiling of exosomes derived from human pulmonary artery smooth muscle cells under PDGF stimulation

| PRJNA607447 | ENA

Growth factor stimulation of cardiomyocytes induces changes in the transcriptional contents of secreted exosomes

Project description:To study if the transcriptional content in exosomes derived from unntreated and growth factor treated cultured cardiomyocytes (HL-1) differ and if so, can this difference be explained.This is results for the cells.The exosome reults can be find at GSE40503. 4 control untreated samples, 4 TFG-beta2 cardiomyocyte treated samples and 4 PDGF BB cardiomyocyte were studied., this reults are from the corresponding cells (that released the exosomes), used for data filtering of the exsomedata. The exosome reults can be find at GSE40503.

2013-06-20 | E-GEOD-45207 | biostudies-arrayexpress

SILAC analysis of PDGF-treated primary bladder smooth muscle cells

Project description:SILAC analysis of human primary bladder smooth muscle cells treated with platelet-derived growth factor for 0, 4, and 24 h. Platelet-derived growth factor-BB (PDGF-BB) is a mitogen and motogen that has been implicated in the proliferation, migration and synthetic activities of smooth muscle cells (SMC) that characterize pathologic tissue remodeling in hollow organs. To explore the signals induced by PDGF on a global scale, we performed expression profiling and quantitative proteomics analysis of PDGF-treated human visceral SMC. 1695 genes and 241 proteins were identified as differentially expressed in PDGF-treated primary bladder SMC versus non-treated cells. Analysis of gene expression data revealed MYC, JUN, EGR1, MYB and RUNX1 as the transcription factors most significantly networked with upregulated genes; DDIT3, NFAT5, and SOX5 were most networked with downregulated genes. For protein identification and quantification, raw mass spectrometric data were analyzed with MaxQuant software (version 1.0.13.13). The parameters were set as follows. In the Quant module, SILAC triplets was selected; oxidation (M) and acetyl (Protein N-term) were set as variable modification; carbamidomethyl (C) was set as fixed modification; concatenated IPI human database (version 3.52) (74,190 forward sequences and 74,190 reverse sequences) was used for database searching; all other parameters were default. Tandem mass spectra were searched by Mascot (version 2.2.0.4) (Matrix Science, Boston, MA). In the Identify module, all parameters were default, except that maximal peptide posterior error probability was set as 0.05. False discovery rates for protein and peptide identifications were both set at 0.01.

2014-08-04 | PXD000624 | Pride

Pulmonary artery banded rat heart

Project description:Changes in juvenile rat heart with pulmonary artery banding

2022-05-20 | PXD027427 | Pride

Human pulmonary artery smooth muscle cells under hypoxia

Project description:Human pulmonary artery smooth muscle cells under hypoxia

| PRJNA485330 | ENA

microRNA profiling of human pulmonary artery smooth muscle cells under hypoxia

Project description:Analysis of hypoxia-exposed human pulmonary artery smooth muscle cells to identify the commonly regulated microRNAs by hypoxia. Results provide insight into the regulatory mechanism of hypoxic responses in vascular smooth muscle cells.

2019-09-25 | GSE118361 | GEO

Transcriptional profiling of human pulmonary artery smooth muscle cells under hypoxia

Project description:Analysis of hypoxia-exposed human pulmonary artery smooth muscle cells to identify the commonly regulated genes by hypoxia. Results provide insight into the regulatory mechanism of hypoxic responses in vascular smooth muscle cells.

2019-09-25 | GSE118362 | GEO

Microarray to identify miR-143 targets in pulmonary artery smooth muscle cells.

Project description:Pulmonary artery smooth muscle cells were either mock transfected, transfected with scramble control or transfected with pre-miR-143. After 24 hours the cells were harvested with Qiazol and processed for a microarray experiment. The experiment was performed in order to identify potential targets of miR-143.

2015-06-26 | E-MTAB-3567 | biostudies-arrayexpress

Human fibroblast stimulation with PDGF-BB or b-FGF

Project description:We analyzed gene expression in human fibroblasts stimulated by platelet-derived growth factor-BB (PDGF-BB) or basic fibroblast growth factor (bFGF) for 1h and 24h. The results of two independent experiments were merged. SAM analysis identified 116 relevant probe sets. Hierarchical clustering of these probe sets showed divergent early gene regulation by PDGF and FGF but overlapping late response. We first analyzed genes commonly regulated by PDGF-BB and b-FGF more than 2 fold after 24h of stimulation and we found that these two growth factors repressed FOXO. We then focused on the early gene expression response induced by both growth factors. We performed a fold change analysis and found 114 probe sets regulated by PDGF-BB and 42 probe sets regulated by b-FGF, 37 of which were shared between the two gene lists . Keywords: Time course, cell Treatment comparison

2009-03-16 | GSE14256 | GEO