Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell transcriptome from aplastic anemia (AA) and healthy donors [Full-length RNA-seq]

ABSTRACT: Transcriptome analysis of hematopoietic stem and progenitor cells (HSPCs) from aplastic anemia (AA) and healthy donors

ORGANISM(S): Homo sapiens

PROVIDER: GSE145531 | GEO | 2021/03/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	GSE145531_Full_length_SingleCell_counts.txt.gz	Txt

Items per page:

1 - 2 of 2

Similar Datasets

Single cell transcriptome from aplastic anemia (AA) and healthy donors [3' RNA-seq]

Project description:Transcriptome analysis of hematopoietic stem and progenitor cells (HSPCs) and T cells collected from bone marrow and peripheral blood of healthy donors and aplastic anemia patients untreated or response to immunosuppressive therapy.

2021-03-31 | GSE145668 | GEO

Bulk RNA-seq of aplastic anemia (AA) and healthy donors

Project description:Bulk RNA-seq data of Lin-CD34+ hematopoietic stem and progenitor cells derived from bone marrow of healthy donors and untreated aplastic anemia patients

2021-03-31 | GSE165870 | GEO

Single cell transcriptome from aplastic anemia (AA) and healthy donors [Full-length RNA-seq]

Project description:Single cell transcriptome from aplastic anemia (AA) and healthy donors [Full-length RNA-seq]

| PRJNA607512 | ENA

Bulk RNA-seq of aplastic anemia (AA) and healthy donors

Project description:Bulk RNA-seq of aplastic anemia (AA) and healthy donors

| PRJNA698389 | ENA

Transcriptome analysis of HSPCs and T cells from aplastic anemia (AA) and healthy donors

Project description:Transcriptome analysis of HSPCs and T cells from aplastic anemia (AA) and healthy donors

| PRJNA607953 | ENA

Transcriptome analysis of HLA-DR(+) HSPCs and HLA-DR(-) HSPCs in aplastic anemia patients

Project description:HLA-DR-lacking HSPCs [HLA-DR(-) HSPCs] were detected in aplastic anemia (AA) patients with HLA-DR15. HLA-DR(-) HSPCs may evade the attack by CD4+ T-cells recognizing the autoantigen presented by HLA-DR15. The goal of this study is to clarify the immune escape mechanisms from antigen-specific T-cells by comparing the trranscriptome profile of HLA-DR(+) HSPCs and HLA-DR(-) HSPCs.

2022-05-31 | GSE183768 | GEO

Single-cell characterization of immune aplastic anemia reveals convergence of adaptive and innate immunity: in vitro

Project description:Immune aplastic anemia (AA) is a life-threatening bone marrow (BM) failure disorder driven by an autoimmune T cell attack against the hematopoietic stem and progenitor cells (HSPCs). However, the autoantigen targets and the role of other immune cells are unknown. Here, by combining scRNA+TCRαβ-seq, TCRβ-seq, flow cytometry and plasma cytokine profiling of >250 patients with AA, >250 patients with other hematological disorders and >1,000 healthy controls, we identify NK cells and CD8+ TEMRA cells expressing NK receptors with AA-associated TCRB-seq motifs as the most dysregulated immune cell populations in AA BM. Functional co-culture experiments with scRNA-TCRαβ-seq readout utilizing primary HSPCs and immune cells (in total 55 conditions) provide evidence that NK cells alone cannot kill HSPCs, but sensitize them to CD8+ T cell mediated killing. Furthermore, HSPCs induce strong activation of T cell clones with CD8+ TEMRA NK-like phenotype and AA-associated TCR motifs. To conclude, our results reveal convergent evolution of innate and adaptive immune cells in AA, where NK cells support CD8+ T cell mediated autoimmunity.

2024-12-12 | E-MTAB-13484 | biostudies-arrayexpress

Single-cell characterization of immune aplastic anemia reveals convergence of adaptive and innate immunity: in vivo

2024-12-12 | E-MTAB-13485 | biostudies-arrayexpress

Single cell transcriptome from aplastic anemia (AA) and healthy donors [3' RNA-seq]

Project description:Single cell transcriptome from aplastic anemia (AA) and healthy donors [3' RNA-seq]

| PRJNA608064 | ENA

MicroRNA expression data from bone marrow T cells of acquired aplastic anemia patients and healthy individuals

Project description:Acquired aplastic anemia (AA) is an immune-mediated disease with active destruction of hematopoietic stem and progenitor cells by the cytotoxic T-cells in bone marrow. Aberrant expression of microRNAs in T-cells results in development of some autoimmune diseases.Screening the potential miRNAs which may play regulatory role in T cells of AA is meaningful to explore the mechanism of AA. We used microarrays to screen the differential expression pattern of miRNAs in T cells of aplatic anemia patients to find the potential regulatory miRNAs.

2017-01-01 | GSE82095 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data