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Comparison of expression in PAM765-transgenic vs GUS-transgenic Arabidopsis

ABSTRACT: PAM765 encodes the secretory protein of a phytopathogenic bacterium, Candidatus Phytoplasma asteris. To investigate the influence of PAM765 expression on the transcription profile of a host plant, we identified differences in the gene expression profiles between PAM765-transgenic and GUS-transgenic Arabidopsis plants using microarray analysis.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE14564 | GEO | 2009/07/30

SECONDARY ACCESSION(S): PRJNA111567

REPOSITORIES: GEO

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Comparison of expression in PAM765-transgenic vs GUS-transgenic Arabidopsis

Project description:PAM765 encodes the secretory protein of a phytopathogenic bacterium, Candidatus Phytoplasma asteris. To investigate the influence of PAM765 expression on the transcription profile of a host plant, we identified differences in the gene expression profiles between PAM765-transgenic and GUS-transgenic Arabidopsis plants using microarray analysis. Three independent transgenic lines were harvested from 2-week-old tengu- or GUS-transgenic plants, and were used for microarray analysis.

2010-04-09 | E-GEOD-14564 | biostudies-arrayexpress

Transcription profiling of Medicago sativa MtTTG1 over-expression transgenic alfalfa gene

Project description:Transcriptomics analysis of transgenic alfalfa (TTG1) versus gus control (GUS) in the cultivar R2336.

2009-08-14 | E-MEXP-1759 | biostudies-arrayexpress

Candidatus Phytoplasma solani mleP1 gene

Project description:Candidatus Phytoplasma solani mleP1 gene encodes a 2-Hydroxycarboxylate transporter

| PRJEB24172 | ENA

Phytoplasma-responsive small RNAs in Mexican lime trees

Project description:Background: Witches’ broom disease of Mexican lime (Citrus aurantifolia L.), which is caused by the phytoplasma “Candidatus Phytoplasma aurantifolia”, is a devastating disease that results in significant economic losses. Plants adapt to abiotic stresses by regulating gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) are a recently identified family of molecules that regulate plant responses to environmental stresses through post-transcriptional gene silencing. Methods: Using a high-throughput approach to sequence small RNAs, we compared the expression profiles of miRNAs in healthy Mexican lime trees and in plants infected with “Ca. Phytoplasma aurantifolia”. Results: Our results demonstrated the involvement of different miRNAs in the response of Mexican lime trees to infection by “Ca. Phytoplasma aurantifolia”. We identified miRNA families that are expressed differentially upon infection with phytoplasmas. Most of the miRNAs had variants with small sequence variations (isomiRs), which are expressed differentially in response to pathogen infection. Conclusions: It is likely that the miRNAs that are expressed differentially in healthy and phytoplasma-infected Mexican lime trees are involved in coordinating the regulation of hormonal, nutritional, and stress signalling pathways, and the complex interactions between them. Future research to elucidate the roles of these miRNAs should improve our understanding of the level of diversity of specific plant responses to phytoplasmas.

2013-03-09 | GSE44279 | GEO

Phytoplasma-responsive small RNAs in Mexican lime trees

Project description:Background: WitchesM-bM-^@M-^Y broom disease of Mexican lime (Citrus aurantifolia L.), which is caused by the phytoplasma M-bM-^@M-^\Candidatus Phytoplasma aurantifoliaM-bM-^@M-^], is a devastating disease that results in significant economic losses. Plants adapt to abiotic stresses by regulating gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) are a recently identified family of molecules that regulate plant responses to environmental stresses through post-transcriptional gene silencing. Methods: Using a high-throughput approach to sequence small RNAs, we compared the expression profiles of miRNAs in healthy Mexican lime trees and in plants infected with M-bM-^@M-^\Ca. Phytoplasma aurantifoliaM-bM-^@M-^]. Results: Our results demonstrated the involvement of different miRNAs in the response of Mexican lime trees to infection by M-bM-^@M-^\Ca. Phytoplasma aurantifoliaM-bM-^@M-^]. We identified miRNA families that are expressed differentially upon infection with phytoplasmas. Most of the miRNAs had variants with small sequence variations (isomiRs), which are expressed differentially in response to pathogen infection. Conclusions: It is likely that the miRNAs that are expressed differentially in healthy and phytoplasma-infected Mexican lime trees are involved in coordinating the regulation of hormonal, nutritional, and stress signalling pathways, and the complex interactions between them. Future research to elucidate the roles of these miRNAs should improve our understanding of the level of diversity of specific plant responses to phytoplasmas. Small mRNA profiles of healthy (H) and Phytoplasma-infected Mexican lime trees were generated by deep sequencing, six replicate, using Illumina Hiseq2000

2013-03-09 | E-GEOD-44279 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Comparison of expression in PAM765-transgenic vs GUS-transgenic Arabidopsis

| PRJNA111567 | ENA

Candidatus Phytoplasma

Project description:Candidatus Phytoplasma overview

| PRJNA187009 | ENA

Project description:Candidatus Phytoplasma solani

| PRJNA825361 | ENA

Project description:Candidatus phytoplasma mali

| PRJEB33432 | ENA

Overexpression of MtABI3 in transgenic Medicago truncatula roots

Project description:ABI3 is a B3-domain transcription factor that acts as a master regulator of seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Medicago hairy roots were generated using Agrobacterium rhizogenes transformed with the genomic sequence of the ABI3 gene of Medicago. Using the Medicago NimbleGen chip, a transciptomic analysis was performed to identify differentially expressed genes compared to the GUS expressed control. Two-condition experiment, GUS versus genomic. Biological replicates: 3 control (35S::GUS), 3 treatment (35S::Genomic ABI3 sequence), independently grown and harvested. One replicate per array.

2013-02-14 | E-GEOD-44291 | biostudies-arrayexpress

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