Transcriptome analysis of tumor infiltrating NK and T cells in CT26-bearing BALB/C mice

ABSTRACT: CT26 tumors were implanted subcutaneously into syngeneic BALB/C mice and allowed to grow for 15-25 days. Tumors were collected, mechanically dissociated, and immune cells enriched using Percoll gradient. Cells were then stained with viability dye, CD45, CD3, and NKp46 to FACS sort for T cells (Live/CD45+/CD3+/NKp46-) and NK cells (Live/CD45+/CD3-/NKp46+). Isolated tumor infiltrating NK and T cells were then processed for RNA sequencing analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE145837 | GEO | 2020/06/01

REPOSITORIES: GEO