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Comparative transcriptomic analysis of subcutaneous adipose tissue from local pig breeds

ABSTRACT: The aim of this work was to explore the genome function of AL and BI pig breeds to better understand the underlying physiological mechanisms associated with body fat accretion and lean muscle tissue deposition and therefore, meat quality of these breeds. Dorsal subcutaneous fat samples were collected at slaughter from adult AL and BI pigs (n=4 for each genotype) with ~150kg body weight. Total RNA was obtained and sequenced for transcriptome analysis and a bioinformatic analysis using the DESeq2 R package was implemented. A total of 458 differentially expressed genes (q-value<0.05) were identified, with 263 found overexpressed in AL and 195 in BI, including 47 novel genes. Several key genes involved in de novo fatty acid biosynthesis, elongation and desaturation were consistently found upregulated in AL such as acly, fasn, me1, elovl6 and scd. A functional enrichment analysis of the candidate genes was performed using Ingenuity Pathway Analysis, which revealed GH signalling, HOTAIR regulatory pathway, AMPK and IGF-1 signalling among the top canonical pathways associated to the BI. Potential upstream regulators such as SCAP, MED1, SREBF2 and PPARG were found activated in AL, suggesting increased adipogenesis and cholesterol synthesis. Regulators found in the BI included INSIG1, INSIG2 and FOXO1 associated with lipogenesis inhibition, and MEF2C and PDGFB, related to myogenesis, cell proliferation and differentiation.

ORGANISM(S): Sus scrofa

PROVIDER: GSE145956 | GEO | 2020/05/26

REPOSITORIES: GEO

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